DIAGNOSIS OF CANINE DEMODICOSIS BY INDIRECT- ELISA IN HYDERABAD OF TELANGANA STATE

FATIMA LUBNA¹, G.S. SREENIVASAMURTHY²*, M. UDAYAKUMAR^3
1Department of Parasitology, College of Veterinary Science, Rajendranagar, 500030, Hyderabad, Telangana
²Department of Parasitology, College of Veterinary Science, Rajendranagar, 500030, Hyderabad, Telangana
³Department of Parasitology, College of Veterinary Science Korutla, Karimnagar, 505326, Telangana
* Corresponding Author : gssmurthy26@rediffmail.com

Received : 28-12-2016     Accepted : 03-01-2017     Published : 12-01-2017
Volume : 9     Issue : 2       Pages : 3663 - 3665
Int J Agr Sci 9.2 (2017):3663-3665

Keywords : ELISA, Canine Demodicosis, Demodicosis
Academic Editor : Dr PV Rao
Conflict of Interest : None declared
Acknowledgements/Funding : We are thankful to Dean, Faculty of Veterinary Sciences of PVNR TVU for providing the financial support to conduct this research study and the fellowship to continue the Master’s programme in the University
Author Contribution : None declared

Canine demodicosis is an inflammatory, non pruritic and non contagious condition is characterized by the presence of numerous mites on the skin. Skin scraping, trichoscopy and skin biopsy are the common diagnostic methods. The existence of subclinical and asymptomatic infection in the skin makes it difficult for veterinarians to diagnose the infection. Hence the present study was aimed to develop a laboratory standardized Indirect ELISA for diagnosis and seroprevalence of canine demodicosis in and around Hyderabad. Demodex canis antigen concentration was optimized at 0.5 µg per well of ELISA nunc plate against 1:50 anti- Demodex positive sera and 1:5000 dilution of Rabbit anti-dog IgG HRPO conjugate. Out of 88 samples collected from animal birth control centers 17 were positive (19.31 %) and out of 72 samples from pet clinics 22 were positive (30.55 %) with Indirect ELISA. The overall sensitivity of test was 70.58% and specificity was 76.92%.