INCIDENCE OF METHICILLIN RESISTANT Staphylococcus aureus IN THE MILK OF SURTI GOATS

S.A. PATEL¹*, C.V. SAVALIA², RAJEEV KUMAR³, MARTINA A. GAMIT⁴, SHRUTI NAIR⁵, R.K. PATEL⁶, N.G. PATEL^7
1Department of Veterinary Public Health & Epidemiology, Vanbandhu College of Veterinary Science and Animal Husbandry, Navsari Agricultural University, Navsari, 396 450, Gujarat, India
²Department of Veterinary Public Health & Epidemiology, Vanbandhu College of Veterinary Science and Animal Husbandry, Navsari Agricultural University, Navsari, 396 450, Gujarat, India
³Department of Veterinary Public Health & Epidemiology, Vanbandhu College of Veterinary Science and Animal Husbandry, Navsari Agricultural University, Navsari, 396 450, Gujarat, India
⁴Department of Livestock Products Technology, Vanbandhu College of Veterinary Science and Animal Husbandry, Navsari Agricultural University, Navsari, 396 450, Gujarat, India
⁵Department of Livestock Products Technology, Vanbandhu College of Veterinary Science and Animal Husbandry, Navsari Agricultural University, Navsari, 396 450, Gujarat, India
⁶Department of Veterinary Public Health & Epidemiology, Vanbandhu College of Veterinary Science and Animal Husbandry, Navsari Agricultural University, Navsari, 396 450, Gujarat, India
⁷Department of Livestock Products Technology, Vanbandhu College of Veterinary Science and Animal Husbandry, Navsari Agricultural University, Navsari, 396 450, Gujarat, India
* Corresponding Author : sagar.2008.patel@gmail.com

Received : 17-12-2016     Accepted : 05-01-2017     Published : 12-01-2017
Volume : 9     Issue : 2       Pages : 3637 - 3639
Int J Agr Sci 9.2 (2017):3637-3639

Keywords : Methicillin-Resistant Staphylococcus aureus (MRSA), Antibiotic resistant, Surti Goat, mecA gene
Academic Editor : Ishwar Bhabhor Aminor, Dr Amit Rathod
Conflict of Interest : None declared
Acknowledgements/Funding : The authors are highly thankful to the Dean, Vanbandhu College of Veterinary science and A.H. for financial assistance and research facilities to conduct this research work
Author Contribution : all author equally contributed

Staphylococcus aureus is found pathogenic to man and animals and it is responsible for food borne outbreaks, too. The goat milk is gaining popularity due to many health benefits on human health. To know carriage of Staph. aureus in the milk of Surti goats reared in Livestock Research Station, NAU, Navsari as well as from two rural goat rearing pockets in southern Gujarat, i.e., Amalsad (Dist. Navsari) and Dharampur (Dist. Valsad), the present investigation was carried out. All the 37 isolates S. aureus isolates were subjected to antibiotic susceptibility testing against 9 selected antibiotics by agar disc diffusion method. The results showed S. aureus isolates were resistant to Cephalexin (62.16%), Methicillin (40.54%), Ampicillin (18.92%) and Kanamycin (2.70 %). All Methicillin Resistant Staphylococcus aureus (MRSA) strains identified by antibiotic sensitivity test, were subjected to PCR to detect methicillin resistant gene mecA. Agarose Gel electrophoresis revealed single compact band of 533 bp, indicative of successful amplification of target mecA gene from the genomic DNA of S. aureus. Out of 37 S. aureus, 15 isolates amplified 533 bp of mecA gene, result show that 40.54 per cent S. aureus isolates carried mecA gene.