MOLECULAR DETECTION AND IDENTIFICATION OF TOMATO LEAF CURL NEW DELHI VIRUS ASSOCIATED WITH YELLOW MOSAIC DISEASE OF RIDGE GOURD (Luffa accutangula L.) BASED ON COAT PROTEIN GENE

MANJUNATH S. HURAKADLI¹*, K.T. RANGASWAMY², S. BASAVARAJ³, M. BHAGYASHREE⁴, N. NAGARAJU⁵, H.A. PRAMEELA^6
1Department of Plant pathology, University of Agricultural Sciences, GKVK, Bengaluru, Karnataka 560065, India
²Department of Plant pathology, University of Agricultural Sciences, GKVK, Bengaluru, Karnataka 560065, India
³Department of Plant pathology, University of Agricultural Sciences, GKVK, Bengaluru, Karnataka 560065, India
⁴Department of Plant pathology, University of Agricultural Sciences, GKVK, Bengaluru, Karnataka 560065, India
⁵Department of Plant pathology, University of Agricultural Sciences, GKVK, Bengaluru, Karnataka 560065, India
⁶Department of Plant pathology, University of Agricultural Sciences, GKVK, Bengaluru, Karnataka 560065, India
* Corresponding Author : mshurakadli@gmail.com

Received : 24-10-2016     Accepted : 12-12-2016     Published : 18-12-2016
Volume : 8     Issue : 61       Pages : 3444 - 3449
Int J Agr Sci 8.61 (2016):3444-3449

Keywords : Tomato leaf curl New Delhi virus (ToLCNDV), Yellow mosaic disease, Ridge gourd, Coat protein gene
Conflict of Interest : None declared
Acknowledgements/Funding : I am highly thankful to Division of Plant Pathology, University of Agricultural Sciences, Bengaluru, India, for providing me with all the required facilities to complete my research and also greatly thankful to ICAR (Indian Council of Agricultural research, New Delhi) for providing Junior Research Fellowship
Author Contribution : None declared

A severe yellow mosaic disease on ridge gourd with a significant disease incidence was observed during the survey at different locations of southern dry zone of Karnataka in the year 2013-2014. The disease consisted of yellow mosaic accompanied with slight curling of leaves and the whitefly (Bemisia tabaci) population was also observed in the field during survey. The characteristic yellow mosaic disease symptoms and whitefly population indicated the possibility of begomovirus infection. PCR was carried out using the total DNA isolated from infected leaf samples and a pair of begomovirus specific primers which resulted in the expected size (~750 bp) amplicon indicated the presence of begomovirus. For further identification of the begomovirus, the PCR amplicons were cloned and sequenced. The sequence data analysis revealed highest of 93-92% similarities with several isolates of Tomato leaf curl New Delhi virus (ToLCNDV) at nucleotide levels. The phylogenetic analysis also showed closest relationships of the causal virus with various variants of ToLCNDV. Based on highest sequence similarities and closest relationships with ToLCNDV, the virus isolated from ridge gourd was considered as an isolate of Tomato leaf curl New Delhi virus.