A RAPID METHOD FOR RICE GENOMIC DNA EXTRACTION WITHOUT LIQUID NITROGEN AND PHENOL

M.Z. SHAMIM¹*, H. MANZAR², V.K. SHARMA^3
1Department of Agricultural Biotechnology & Molecular Biology, Rajendra Agricultural University, Pusa, 848125, Bihar, India
²Department of Agricultural Biotechnology & Molecular Biology, Rajendra Agricultural University, Pusa, 848125, Bihar, India
³Department of Agricultural Biotechnology & Molecular Biology, Rajendra Agricultural University, Pusa, 848125, Bihar, India
* Corresponding Author : mzshamim85@gmail.com

Received : 28-08-2016     Accepted : 04-09-2016     Published : 30-10-2016
Volume : 8     Issue : 52       Pages : 2528 - 2530
Int J Agr Sci 8.52 (2016):2528-2530

Keywords : CTAB method, DNA extraction, PCR, Rice
Academic Editor : Lokesh Bora
Conflict of Interest : None declared
Acknowledgements/Funding : Authors are grateful to the Department of Biotechnology (DBT) Government of India for financial support during the study period. We are also thankful to Dr. V.K. Shahi, Dean Faculty of Basic Sciences & Humanities (FBS&H), Rajendra Agricultural University, Pusa, Bihar, India for providing laboratory facilitation for this study.
Author Contribution : None declared

A rapid, safe and inexpensive method for good quality DNA extraction from rice leaves is the basic requirement for molecular breeding in rice. Here we described a faster, safe and inexpensive method for genomic DNA extraction from rice leaves after modification of CTAB method. In the described method liquid nitrogen and Phenol is fully avoided for DNA extraction. This method yields approximately 110 ng/µl-420 ng/µl of DNA from 300 mg of fresh leaves tissue, depending on rice varieties and grinding of leaf samples. It can be completed within 4-5 hrs. The extracted DNA is stable and it can be used for DNA fingerprinting, quantitative traits loci analysis, marker-assisted selection, and genetic diversity analysis.