ENHANCED PRODUCTION OF XYLANASE FROM WHITE ROT FUNGUS C. cinerea RM-1 NFCCI-3086BY STANDARDIZATION OF NUTRIENT SALT SOLUTION

POONAM MAAN¹*, DHARM DUTT², A.K. VIDYARTHI^3
1Department of Paper Technology, Indian Institute of Technology Roorkee, Saharanpur, 247 001, India
²Department of Paper Technology, Indian Institute of Technology Roorkee, Saharanpur, 247 001, India
³Central Pollution Control Board (Ministry of Environment and Forest), Parivesh Bhawan, East Arjun Nagar, New Delhi, 110032
* Corresponding Author : prm.iitr@gmail.com

Received : 11-08-2016     Accepted : 19-08-2016     Published : 30-10-2016
Volume : 8     Issue : 52       Pages : 2390 - 2393
Int J Agr Sci 8.52 (2016):2390-2393

Keywords : Nutrient salt solution, Xylanase, C. cinerea, Wheat bran, Corn cob
Academic Editor : Dr Yogendra Singh
Conflict of Interest : None declared
Acknowledgements/Funding : Financial support from Ministry of Human Resource Development (MHRD), New Delhi, India, is gratefully acknowledged
Author Contribution : None declared

One variable at a time approach has been employed to evaluate the effect of different components of NSS medium associated with xylanase production by the fungus C. cinerea RM-1 NFCCI 3086 using wheat bran and corn cob as substrate. Different sources of carbon, organic and inorganic nitrogen and surfactant were assessed in different concentrations for their significance on xylanase production under solid state fermentation. As a result of optimization, (NH4)2SO4 (3 g/L) was proved to be better in comparison to NH4Cl (4 g/L). Tween 80 (0.1 g/L) was also show good improvement in xylanase production. The xylanase activity was improved by 18.0% under optimized NSS medium (864.8 IU/mL) as compared to unmodified NSS medium (708.9 IU/mL).This standardized NSS medium shows very good enhancement in xylanase activity in a cost effective manner and can be used for further optimization studies of xylanase production.