EFFECT OF HOST PLANT EXTRACTS ON NUTRIENT MEDIA FOR DIFFERENT GROWTH CHARACTER AND SPORULATION OF PLANT PATHOGENS

JAYASHREE BHATTACHARJEE¹*, DEBASHRE BHATTACHARJEE², S.G. BORKAR³, GANESHKUMAR D. REDE^4
1Department of Plant Pathology, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, Nadia, 741252, West Bengal
²ICAR Research Complex for NEH Region, Tripura
³Post Graduate Institute, Mahatma Phule Krishi Vidyapeeth, Rahuri, Ahmednagar, 413722, Maharashtra
⁴Post Graduate Institute, Mahatma Phule Krishi Vidyapeeth, Rahuri, Ahmednagar, 413722, Maharashtra
* Corresponding Author : jayashreebhatt89@gmail.com

Received : 05-07-2016     Accepted : 11-07-2016     Published : 21-10-2016
Volume : 8     Issue : 49       Pages : 2086 - 2089
Int J Agr Sci 8.49 (2016):2086-2089

Keywords : Pathogen, Mycelial growth, Colony character, Sporulation, Culture media
Academic Editor : Tushar Manohar Ghule, Vikas Kumar Ravat, Rajendra D Solanki
Conflict of Interest : None declared
Acknowledgements/Funding : The authors thankful to Chairman and Research Guide, Dr S.G. Borkar, Associate Dean, Post Graduate Institute, M.P.K.V., Rahuri. Authors express sincere thanks to Dr B.K. Ulmek, Dean, Faculty of Agriculture for permission and providing necessary facilities for research work.
Author Contribution : None declared

An experiment was done to know the effect of leaf extract and root extraction nutrient media for mycelial growth, colony character and sporulation. Observation was recorded after 72. However, maximum number of spores/cm2, mycelial growth was recorded in the nutrient media where leaf and root extract were added. Media rich in nutrient is called culture media which is used for the growth and sporulation of micro-organism. A survey was carried out on some gelling agent like Tapioca and Agar for the growth of plant pathogens such as Fusarium oxysporum, Alternaria alternata, Helminthosporium sp, Cercospora sp, Sclerotinia sp were isolated from different plant. Potato-Dextrose-Agar (PDA) and Tapioca-Potato-Sugar-Agar (TPSA) media were used to maintain the culture, to screen their suitability for culturing fungi. The root and leaf extract of their respective plant parts were added the Tapioca-potato-sugar-agar (TPSA) and potato dextrose agar (PDA) media to check the efficacy of the pathogen to their respective host plant. After incubation at 27±1OC, growth rate measured and sporulation counted for fungal organism. The growth rates and sporulation of all the organisms in TPSA media and TPSA Media with leaf extract were observed. TPSA Media with leaf extract was significantly higher than those in Potato Dextrose Agar media and TPSA media.