EFFECT OF PYRACLOSTROBIN ON INDUCTION OF PATHOGENESIS RELATED PROTEINS IN BRINJAL (SOLANUM MELONGENA L) AGAINST PHOMOPSIS VEXANS

S. MAHADEVAKUMAR¹, G.R. JANARDHANA²*
¹Mycology and Phytopathology Laboratory, Department of Studies in Botany, University of Mysore, Manasagangotri, Mysore-570 006, Karnataka, India
²Mycology and Phytopathology Laboratory, Department of Studies in Botany, University of Mysore, Manasagangotri, Mysore-570 006, Karnataka, India
* Corresponding Author : grjbelur@gmail.com , mahadevakumars@gmail.com

Received : 30-01-2016     Accepted : 14-02-2016     Published : 14-03-2016
Volume : 8     Issue : 8       Pages : 1080 - 1082
Int J Agr Sci 8.8 (2016):1080-1082

Keywords : Solanum melongena, Phomopsis vexans, Lipoxygenase, Chitinase, Glucanase
Academic Editor : Antonio Santos-rufo, B. Janse Van Rensburg, G. D. Sinniah
Conflict of Interest : None declared
Acknowledgements/Funding : First author (Mahadevakumar, S.) would like to thank Department of Science and Technology, New Delhi for proving INSPIRE Fellowship (IF110422).
Author Contribution : None declared

In the present study, the effect of Pyraclostrobin treatment on induction of pathogenesis related proteins in a susceptible brinjal cultivar (cv. PPL) was studied upon challenge inoculation with Phomopsis vexans_MK2 isolate. Seeds treated with sterile distilled water served as controls. Seeds treated with 0.2% Pyraclostrobin along with respective control seeds (cv. PPL) were raised under nursery bed. Thirty days old seedlings were challenge inoculated with conidial suspension of P. vexans (1 x106 conidia/ml). Seedlings were harvested at regular intervals of 0, 3, 6, 12, 24, 48, 72 & 96 hours of post inoculation and analyzed for activity of pathogenesis related proteins (PRP’s) namely β -1,3-glucanase, chitinase and lipoxygenase, colorimetrically. The results revealed that Pyraclostrobin treatment enhanced the activity of pathogenesis related proteins when compared to control seedlings (water inoculated, challenge inoculated with P. vexans) Significant differences (P - 0.001) in activity of β -1,3-glucanase was observed at 48hpi upon Pyraclostrobin treatment followed by challenge inoculation with P. vexans. An increased chitinase(P-0.03) activity was also recorded at 48-96 hpi upon Pyraclostrobin treatment and lipoxygenase activity was found maximum at 48 hpi. The results indicated thatPyraclostrobinhave a positive effect on the induction and activity of defense related proteins like β -1,3-glucanase, chitinase and lipoxygenase.