ASSESSMENT OF GENETIC DIVERSITY OF PENNISETUM GLAUCUM (L.) R. Br. AND TRITICUM AESTIVUM L. GENOTYPES THROUGH RAPD AND SSR MARKERS

SUDESHNA CHAKRABORTY¹*, N. SASIDHARAN², RUCHI TRIVEDI³, BHUSHAN AHER⁴, D.A. PATEL^5
1Department of Seed Science and Technology, Anand Agricultural University, Anand 388110, Gujarat, India
²Department of Seed Science and Technology, Anand Agricultural University, Anand 388110, Gujarat, India
³Department of Seed Science and Technology, Anand Agricultural University, Anand 388110, Gujarat, India
⁴Department of Seed Science and Technology, Anand Agricultural University, Anand 388110, Gujarat, India
⁵Department of Seed Science and Technology, Anand Agricultural University, Anand 388110, Gujarat, India
* Corresponding Author : schakraborty.bio@gmail.com

Received : 17-12-2015     Accepted : 02-01-2016     Published : 07-01-2016
Volume : 8     Issue : 1       Pages : 947 - 951
Int J Agr Sci 8.1 (2016):947-951

Keywords : RAPD, SSR, PIC, Pearl millet, Wheat, Genetic diversity
Academic Editor : Can Wang, Dr P Sujatha, S. D. Bhawsar, Dr B. P. Bhaskar, Dr. Puspendu Dutta
Conflict of Interest : None declared
Acknowledgements/Funding : None declared
Author Contribution : None declared

The present work deals with the comparison of the level of information provided by RAPD and SSR marker systems for estimating genetic similarities in pearl millet and wheat genomes. For the present investigation sixteen pearl millet and fourteen wheat genotypes were used. The RAPD analysis of pearl millet and wheat genotypes produced 894 and 564 scorable bands respectively. The RAPD primer 22ES10G33 and OPN-7 revealed the highest PIC values of 0.96 and 0.97 for pearl millet and wheat respectively. As in the case of SSR primers CTM25, CTM27 and Xpsmp 2273 gave the highest PIC value of 0.84 for pearl millet and 0.88 for wheat genotypes by Xgwm153. In both the cases i.e, RAPD and SSR, the respective dendrograms developed revealed that the local varieties tend to group together, showing the efficiency of these marker systems in diversity analysis. However, although during the investigation, it was observed that RAPD markers are more efficient than SSR for revealing the latent diversity underlying these crop genotypes, it cannot be generalized in all instances.