ROLE OF PROLINE AND STAY GREEN TRAIT IN IMPROVING PLANT RESISTANCE AGAINST ABIOTIC STRESS IN WHEAT

N.P. SINGH¹*
¹Department of Biotechnology, SVP University of Agriculture and Technology, Meerut, 250110, India
* Corresponding Author : naresh.singh55@yahoo.com

Received : 25-10-2015     Accepted : 07-12-2015     Published : 21-12-2015
Volume : 7     Issue : 14       Pages : 875 - 878
Int J Agr Sci 7.14 (2015):875-878

Keywords : Ethyl Metanesulphonate, Stay green, Biochemical, Proline, Wheat, Chlorophyll, Osmolytes
Academic Editor : Dr A. K Mishra, Dr. Y. Ramakrishna, Dr Krishnamoorthy Ramasamy
Conflict of Interest : None declared
Acknowledgements/Funding : None declared
Author Contribution : None declared

At present, abiotic stresses is emerging as the problem of great concern for the agriculture ecosystem. Under abiotic stresses plants greatly affected at molecular, physiological and biochemical level. Moreover, stay green phenotypes have the capability to maintain longer green leaf area and also helps in triggering the production of osmoprotectants such as proline etc. in stressed environment, which directly results in good yield. Present research was conducted to observe proline accumulation in wheat varieties after treatment with Ethyl Methanesulphonate (EMS) of three concentrations (0.5%, 1.0% and 1.5%) for 90 minutes. The proline content was observed at booting and maturity stage. At booting stage, proline content was varied from 1.45 µmol/gfw in K 7410 to 1.05 µmol/gfw in variety HD 2177 in control plants. Variety K 7410 showed highest proline content ranges from 1.45 to 1.75 µmol/gfw after the three treatments of EMS. At maturity stage, the proline content varied from 1.05 to 2.12 µmol/gfw in control plants, 1.04 to 2.25 µmol/gfw after treatment one(0.5%), 1.00 to 2.36 µmol/gfw after treatment second(1%) and 1.00 to 2.65 µmol/gfw after treatment third(1.5%) of EMS. Whereas, variety HD 2177 and K 68 shows significant decreased level of proline content after three treatments. On the whole, these ten wheat varieties showed decreased level of proline after three treatments of EMS except K 7410 at both the stages. Such results indicates that treatment of EMS help in developing such wheat varieties which may perform better under abiotic stress conditions such as drought, salt etc.