THE SEARCH FOR THE R1-NJ MAIZE GENE MARKER ENABLING THE SELECTION OF IN VIVO PRODUCED HAPLOID FORMS

S. MIKOLAJCZYK¹, A. TOMKOWIAK², D. WEIGT³, Z. BRODA⁴, D. KURASIAK POPOWSKA⁵*
¹Department of Genetics and Plant Breeding, Poznan University of Life Sciences, Dojazd 11, 60-632 Poznan, Poland
²Department of Genetics and Plant Breeding, Poznan University of Life Sciences, Dojazd 11, 60-632 Poznan, Poland
³Department of Genetics and Plant Breeding, Poznan University of Life Sciences, Dojazd 11, 60-632 Poznan, Poland
⁴Department of Genetics and Plant Breeding, Poznan University of Life Sciences, Dojazd 11, 60-632 Poznan, Poland
⁵Department of Genetics and Plant Breeding, Poznan University of Life Sciences, Dojazd 11, 60-632 Poznan, Poland
* Corresponding Author : popowska@up.poznan.pl

Received : 28-09-2015     Accepted : 19-10-2015     Published : 14-11-2015
Volume : 7     Issue : 9       Pages : 698 - 702
Int J Agr Sci 7.9 (2015):698-702

Keywords : Maize, in vivo, Inductor, Haploid, RAPD
Academic Editor : Niravkumar Ashokbhai Delvadiya, Md Atikur Rahman, M. Jankulovska, Cyril Kolese Atung, Dr Lidia Skuza, Mihaela Ivanova, Gyan Prakash Mishra
Conflict of Interest : None declared
Acknowledgements/Funding : None declared

The aim of this study was an attempt to identify the polymorphic product, which is characteristic of haploid maize lines, produced by inductor fertilisation. The plant material used for the research consisted of 6 haploid lines of maize produced by pollination with an inductor, which had an anthocyanin marker on the seeds, and it also consisted of 3 diploid forms. The ploidy level of the lines of suspected haploids under analysis was verified by means of a flow cytometer and cytological investigations. 280 oligonucleotide primers were tested in the research. On the basis of RAPD-PCR reactions one characteristic PCR product differentiating haploid from diploid plants was identified.. Probably this product is related with the R1–nj gene expression. The gene is responsible for generation of the anthocyanin pigment in the embryo and endosperm of haploid maize forms and it may be the genetic marker of the trait under analysis.