GENETIC PURITY ASSESSMENT OF RICE VARIETIES USING MICROSATELLITE MARKERS

R. KUMAR¹*, P. KUMAR², V.K. SHARMA³, R. KUMARI⁴, K. NEHA^5
1Department of Agricultural Biotechnology & Molecular Biology, Faculty of Basic Sciences and Humanities, Rajendra Agricultural University, Samastipur - 848 125, Bihar, India.
²Department of Agricultural Biotechnology & Molecular Biology, Faculty of Basic Sciences and Humanities, Rajendra Agricultural University, Samastipur - 848 125, Bihar, India.
³Department of Agricultural Biotechnology & Molecular Biology, Faculty of Basic Sciences and Humanities, Rajendra Agricultural University, Samastipur - 848 125, Bihar, India.
⁴Department of Agricultural Biotechnology & Molecular Biology, Faculty of Basic Sciences and Humanities, Rajendra Agricultural University, Samastipur - 848 125, Bihar, India.
⁵Department of Plant Breeding and Genetics, Bihar Agricultural University, Sabour - 813 210, Bihar, India.
* Corresponding Author : ravigeneticss@gmail.com

Received : 04-06-2015     Accepted : 18-09-2015     Published : 01-11-2015
Volume : 7     Issue : 7       Pages : 581 - 586
Int J Agr Sci 7.7 (2015):581-586

Keywords : Microsatellite markers are very useful for the analysis of genetic purity and diversity assessment. Present study was aimed to analyze genetic purity in seed mixture and diversity assessment among different rice varieties. All the 6 (RM 1, RM 242, RM 276, RM285, RM 429, and RM 341) primers used in this investigation were found polymorphic, revealing 28 allelic variants at 9 loci with an average of 3.1 alleles per locus among the 7 rice varieties and their seed mixture tested. A total of 13 shared and 15 unique allelic variants were generated among the varieties and varietal mixture tested. Considering the number of alleles in conjunction with the level of polymorphism detected, these 4 SSR markers (RM 1, RM 429, RM 242 and RM 341) appeared to be more informative primers. Among the primer pairs used for molecular profiling, RM 1, RM 242 and RM 341 appeared to be relatively more useful in revealing comparatively more easily recognizable and predictable pattern of polymorphism for genetic purity testing.
Academic Editor : K.N. Singh, Bishun Deo Pasad, Kumar Amit, Fiyaz R. Abdul
Conflict of Interest : None declared
Acknowledgements/Funding : The authors are thankful to Dr. Md. Shamin. Bihar Agricultural University, sabour, Bihar, India for reviewing the manuscript critically.

Microsatellite markers are very useful for the analysis of genetic purity and diversity assessment. Present study was aimed to analyze genetic purity in seed mixture and diversity assessment among different rice varieties. All the 6 (RM 1, RM 242, RM 276, RM285, RM 429, and RM 341) primers used in this investigation were found polymorphic, revealing 28 allelic variants at 9 loci with an average of 3.1 alleles per locus among the 7 rice varieties and their seed mixture tested. A total of 13 shared and 15 unique allelic variants were generated among the varieties and varietal mixture tested. Considering the number of alleles in conjunction with the level of polymorphism detected, these 4 SSR markers (RM 1, RM 429, RM 242 and RM 341) appeared to be more informative primers. Among the primer pairs used for molecular profiling, RM 1, RM 242 and RM 341 appeared to be relatively more useful in revealing comparatively more easily recognizable and predictable pattern of polymorphism for genetic purity testing.