MICROPROPAGATION OF Capsicum chinense Jacq. cv. LOTA BHOT VIA INDIRECT ORGANOGENESIS

BORA G.¹*, GOGOI H.K.², HANDIQUE P.J.^3
1Division of Biotechnology, Defence Research Laboratory (DRL), Tezpur- 784 001, Assam, India.
²Division of Biotechnology, Defence Research Laboratory (DRL), Tezpur- 784 001, Assam, India.
³Department of Biotechnology, Gauhati University, Guwahati- 781 014, Assam, India.
* Corresponding Author : geetashreejht@gmail.com

Received : 03-05-2014     Accepted : 24-05-2014     Published : 10-07-2014
Volume : 6     Issue : 1       Pages : 384 - 387
Int J Agr Sci 6.1 (2014):384-387
DOI : http://dx.doi.org/10.9735/0975-3710.6.1.384-387

Keywords : Lota Bhot, callusogenesis, In vitro flower induction, In vitro fruit development, Hardening, GA3, AgNO3
Conflict of Interest : None declared
Acknowledgements/Funding : Authors thank the Director, Defence Research Laboratory (DRL), Tezpur, Assam for showing keen interest and providing DRDO fel-lowship. The DRL and Department of Biotechnology (Gauhati Uni-versity) are duly acknowledged for providing necessary facilities a

Capsicum chinense Jacq. is well known for its pungency factor, “capsaicin”. An attempt has been made to maintain the desired pungency of Capsicum chinense Jacq. cv. Lota Bhot through in vitro propagation via callusogenesis from leaf explants under the influence of different plant growth hormones and an additional component, Silver nitrate (AgNO3). The combination of Kinetin (45µML-1) along with 2,4-Dichlorophenoxy Acetic Acid (2,4-D) and AgNO3 at 3.5µML-1 and 35µML-1 respectively was found optimum for callus induction as well as multiple shoot induction whereas maximum shoot length was recorded at Kin (40µML-1), 2,4-D (3.0 µML-1) and AgNO3 (30µML-1). In vitro flower induction and fruit development was most observed under the influence of Gibberellic Acid (GA3) at 30µML-1. Murashige and Skoog (MS) medium fortified with Benzyl Amino Purine (BAP) 1and 2,4-D at 4.0µML-1 and 5.5 µML-1 correspondingly was standardized for maximum induction of roots in vitro. Healthy in vitro regenerated plantlets were acclimatized in the potting substrate containing half strength MS medium enriched with soil, sand, vermicompost and vermiculite in all equal proportion and 78.56% of total acclimatized plants were successfully transferred to the main field.

[1] Agrawal S., Chandra N. and Kothari S.L. (1989) Curr. Sci., 57, 1347-1349.  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[2] Harvell K.P. & Bosland P.W. (1997) HortScience., 32, 1992  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[3] Sanatombi K. & Sharma G.J. (2007) Scientia Horticulturae, 113, 96-99.  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[4] Sanatombi K. & Sharma G.J. (2008) Biologia Plantarum, 52(1), 141-145.  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[5] Christopher T. & Rajam M.V. (1994) Plant Cell Tiss. Org. Cult., 38, 25-29.  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[6] Ramirez-Malagon R. & Ochoa-Alejo N. (1996) Plant Cell Rep., 16, 226-231.  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[7] Bodhipadma K. & Leung D.W.M. (2003) In Vitro Cellular and Developmental Biology - Plant, 39(5), 536-539.  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[8] Kumar A.M. & Nair A.S. (2004) Plant Cell Biotech. Mol. Biol., 5(3&4), 95-100.  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[9] Peddaboina V., Christopher T. & Subhash K. (2006) Scientia Hort., 107(2), 117-122.  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[10] Sanatombi K. & Sharma G.J. (2006) Journal of Food Agricul-tural Environment, 4, 205-208.  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[11] Sharma A., Kumar V., Giridhar P. & Ravishankar G.A. (2008) Electronic Journal of Biotechnology, 11(2), 1-6.  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[12] Ashrafuzzaman M., Hossain M.M., Ismail M.R., Haque M.S., Shahidullah S.M. and Uz-zaman S. (2009) African Journal of Biotechnology, 8(4), 591-596.  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[13] Song J.Y., Sivanesan I, An C.G. & Jeong B.R. (2010) African Journal of Biotechnology, 9(19), 2768-2773.  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[14] Ostroshy M., Moradi K., Nekouei M.K. & Struik P.C. (2011) Asian Journal of Biotechnology, 3(1), 38-45.  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[15] Kehie M., Kumaria S. & Tandon P. (2012) 3 Biotech, 2(1), 31-35.  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[16] Tisserat B. & Galleta D.P. (1995) HortScience, 30, 130-132.  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[17] Rkhis A.C., Maalej M., Messauod S.O. and Drira N. (2006) Afri-can Journal of Biotechnology, 5(22), 2092-2302.  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[18] Ziv M. & Naor V. (2006) Propagation of Ornamental Plants, 6(1), 3-16.  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[19] Beyer E.M. (1976) HortScience, 11, 175-196.  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[20] Murashige T. & Skoog F. (1962) Physiol. Plant, 15, 473-497.  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[21] Kumari M., Patade V.Y. & Ahmed Z. (2012) World Journal of Science and Technology, 2(7), 26-35.  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[22] Giridhar P., Indu E.P., Vijaya R.D. & Ravishankar G.A. (2003) Tropical Science, 43(3), 144-146.  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus