Methicillin resistance of staphylococcal strains remains a public health concern. The present study aims to detect the carriage of the mecA gene by staphylococcal strains and the associated virulence factors. Seventy-eight staphylococcal strains collected from three hospitals in southern Benin were identified in Maldi T of MS. Antibiotic sensitivity was determined by antibiotic disk diffusion on Mueller Hinton-2 medium. The search for the mecA gene, the production of Panton-Valentine PVL, Leucocidin, Toxic shock syndrome toxin TSST and detection of Staphylococcal chromosomal cassette were performed by Real Time - Polymerase Chain Reaction. The results obtained show that Staphylococcus saprophyticus was the most isolated species in the urine (38.4%) followed by Staphylococcus aureus (21.8%), Staphylococcus sciuri (21.8%), Staphylococcus conhii (6, 4%), Staphylococcus heamolyticus (1.3%), Staphylococcus hominis (1.3%) and Staphylococcus xylosus (1.3%). In the cervico-vaginal secretion specimens, Staphylococcus aureus was the most isolated with a proportion of 5.1%. From the study of antibiotic sensitivity, there is a strong resistance of strains to beta-lactams but no resistance to glycopeptides. 24.4% of the staphylococcal strains harbored the mecA gene. Between them, 52.4% of the Staphylococcus aureus strains were carriers of the mecA gene and 41.2% of the Staphylococcus sciuri strains also carried them. 33.3% of strains of Staphylococcus aureus produced PVL and 14.3% of the TSST. Two types of cassette were identified in Staphylococcus aureus ccrB IV (n = 3) and ccrC (n = 4). Only one type of cassette was found in the strains of Staphylococcus sciuri bearing the mecA gene, this is ccrB II (n = 1). It is therefore important to initiate the search for these genes routinely for the proper taking care of patients.

Background: Resistance to broad spectrum beta lactamase mainly those mediated by ESBL’s and MBL’s enzymes is an increasing problem worldwide. Detection of their prevalence and co-existence is essential so as to formulate an effective antibiotic policy and hospital infection control measures. Thus this present study was undertaken to determine the prevalence and co-existence of ESBL & MBL in P.aeruginosa isolates from pus samples. Material and methods: A total of 1100 pus samples were screened, of which 90 isolates of P.aeruginosa were isolated and subjected for ESBL and MBL phenotypic tests. Double disc synergy test and Imipenem (IMP) - EDTA combined disc test were used for their detection respectively. Results: Out of the 90 P.aeruginosa isolated from pus samples, 55(61%) were Cefazidime sensitive and 35(38.8%) were resistant. Of the 35 P.aeruginosa resistant to ceftazidime, DDST detected 16(45.7%).65(72.2%) were Imipenem sensitive and 25(27.7%) were resistant. Of the 25 P.aeruginosa resistant to Imipenem, IMP-EDTA CDT detected 16(64%) of ESBL producers.12(13.3%) showed Resistance to both Imipenem and Ceftazidime and only 6 of the 12 showed Co-existence of ESBL and MBL accounting for 50% of them and 6.6% of the total 90 P. aeruginosa isolates. Conclusion: There is a need for screening tests for detection of not only individual occurrence of different beta lactamases but also their co-existence in the same organism to be made a mandatory routine in all microbiology laboratories.

The present manuscript deals the synthesis of some new di-organocomplexes of group 15 elements especially As, Sb and Bi with 3-amino-6-chloropyridazine. The compounds were synthesized by reported methods and characterized by their melting points, elemental analysis along with IR, NMR spectral analysis to establish the structure of complexes. These compounds were first time screened for their biological efficacy against different pathogenic microbial culture strains. The results are very much promising that few of them compounds are found highly active against biological system.

NTM is increasingly isolated worldwide. Different species are prevalent in different geographic areas around the world1.Treatment of NTM varies depending upon the species isolated and susceptibility profile of the species. So, it is very important to know which species is prevalent in a specific geographical area. In this study we isolated the various NTM species from the clinical samples received in our laboratory and from our study we found that M. avium complex is the most commonly isolated species followed by M. haemophilicum.

Urinary tract infections (UTIs) remain the common infections diagnosed in outpatients as well as hospitalized patients. Knowledge on antimicrobial susceptibility pattern is essential for appropriate therapy. The aim of this study was to describe pathogens frequency and their resistance patterns.5377 samples were received and proceed, out of them 1701 (31.63%) were positive among which the most prevalent were E. coli (44.97%) followed by Klebsiella species. (21.81%), & Pseudomonas species (13.69%). Furthermore, we had a high percentage of samples from people aged 46-60 and aged less than 15, predominantly women. Among the gram-negative enteric bacilli high prevalence of resistance was observed against many antimicrobial agents. This study revealed that E. coli were the predominant bacterial pathogen of UTIs in Ahmedabad, India. This study is useful for clinician in order to improve the empiric treatment and case management of UTIs.