METHICILLIN-RESISTANCE OF Staphylococcus species IN SOUTHERN BENIN: RESISTANCE GENE, VIRULENCE FACTOR ASSOCIATED AND STAPHYLOCOCCAL CHROMOSOMAL CASSETTE DISTRIBUTION

HORNEL KOUDOKPON¹, TAMEGNON VICTORIEN DOUGNON²*, SETONDJI ISLAMIATH KISSIRA³, ALIDAH ANIAMBOSSOU⁴, V. BRICE ARMAND FANOU⁵, FREDERIC LOKO⁶, HONORE SOUROU BANKOLE⁷, M. SEYDINA DIENE⁸, JEAN MARC ROLAIN^9
1Research Unit in Applied Microbiology and Pharmacology of natural substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, 01 PO Box 2009 Cotonou, Benin
²Research Unit in Applied Microbiology and Pharmacology of natural substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, 01 PO Box 2009 Cotonou, Benin
³Research Unit in Applied Microbiology and Pharmacology of natural substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, 01 PO Box 2009 Cotonou, Benin
⁴Research Unit in Applied Microbiology and Pharmacology of natural substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, 01 PO Box 2009 Cotonou, Benin
⁵Research Unit in Applied Microbiology and Pharmacology of natural substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, 01 PO Box 2009 Cotonou, Benin
⁶Research Unit in Applied Microbiology and Pharmacology of natural substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, 01 PO Box 2009 Cotonou, Benin
⁷Research Unit in Applied Microbiology and Pharmacology of natural substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, 01 PO Box 2009 Cotonou, Benin
⁸URMITE, Aix-Marseille University, 27 Bd Jean Moulin, 13385 Marseille Cedex 5, France
⁹URMITE, Aix-Marseille University, 27 Bd Jean Moulin, 13385 Marseille Cedex 5, France
* Corresponding Author : victorien88@hotmail.com

Received : 21-11-2017     Accepted : 12-12-2017     Published : 28-12-2017
Volume : 9     Issue : 12       Pages : 976 - 980
Int J Microbiol Res 9.12 (2017):976-980

Keywords : Public Health, Antimicrobial Resistance, Staphylococcus spp, Molecular diagnosis, Bacteriology
Academic Editor : Gopalakrishnan Reshmi, Garima Kapoor, Shivanna Vijaya
Conflict of Interest : None declared
Acknowledgements/Funding : This study has been done with the support of the Ministry of Higher Education and the Scientific Research in Benin
Author Contribution : All the authors equally contributed to the manuscript

Methicillin resistance of staphylococcal strains remains a public health concern. The present study aims to detect the carriage of the mecA gene by staphylococcal strains and the associated virulence factors. Seventy-eight staphylococcal strains collected from three hospitals in southern Benin were identified in Maldi T of MS. Antibiotic sensitivity was determined by antibiotic disk diffusion on Mueller Hinton-2 medium. The search for the mecA gene, the production of Panton-Valentine PVL, Leucocidin, Toxic shock syndrome toxin TSST and detection of Staphylococcal chromosomal cassette were performed by Real Time - Polymerase Chain Reaction. The results obtained show that Staphylococcus saprophyticus was the most isolated species in the urine (38.4%) followed by Staphylococcus aureus (21.8%), Staphylococcus sciuri (21.8%), Staphylococcus conhii (6, 4%), Staphylococcus heamolyticus (1.3%), Staphylococcus hominis (1.3%) and Staphylococcus xylosus (1.3%). In the cervico-vaginal secretion specimens, Staphylococcus aureus was the most isolated with a proportion of 5.1%. From the study of antibiotic sensitivity, there is a strong resistance of strains to beta-lactams but no resistance to glycopeptides. 24.4% of the staphylococcal strains harbored the mecA gene. Between them, 52.4% of the Staphylococcus aureus strains were carriers of the mecA gene and 41.2% of the Staphylococcus sciuri strains also carried them. 33.3% of strains of Staphylococcus aureus produced PVL and 14.3% of the TSST. Two types of cassette were identified in Staphylococcus aureus ccrB IV (n = 3) and ccrC (n = 4). Only one type of cassette was found in the strains of Staphylococcus sciuri bearing the mecA gene, this is ccrB II (n = 1). It is therefore important to initiate the search for these genes routinely for the proper taking care of patients.

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