The existence of large concern over the increasing resistance to antibiotics for clinically important pathogens which cause varied number of diseases has led to monitor the prevailing resistance pattern of microbes in a study which was carried out in the city of Vijayawada in Andhra Pradesh. In this study, the data of urinary isolates which showed antibiotic resistance patterns were collected from government hospital, multispecialty hospital and a private laboratory. Total 1424 retrospective samples were collected and evaluated for antimicrobial resistance. The samples 676 (47.5%) were positive and 748 (52.5%) were negative to the infection. The antibiotics which showed higher rate of resistance by the urinary isolates were Amoxicillin, Ciprofloxacin, Co-Trimoxazole, Norfloxacin, Nalidixic acid. In contrast, organisms showed susceptibility to Amikacin and then to Azithromycin. Among the gram negative organisms, Escherichia coli was resistance to Amoxicillin (100%), Nalidixic Acid (92.6%), while Klebsiella pneumoniae was found resistance to Amoxicillin (96.3%), Gentamycin (83.3%), Pseudomonas aeruginosa was highly resistance (100%) to Amoxicillin, Ciprofloxacin, Norfloxacin, Nalidixic acid and Co-trimoxazole. Under gram positive organisms Staphylococcus aureus showed resistance to Amoxicillin (72.7%), Nalidixic acid (66.7%) and Enterococcus faecalis was resistance to Nalidixic acid (88.9%) and Norfloxacin (83.3%).

Asparagus racemosus is important medicinal plant of tropical and subtropical India, is a potent phytoestrogen, which is used extensively in the treatment of menopausal symptoms, diarrhea, dyspepsia, and neurodegenerative disorders. The multiple uses of this species have increased its commercial demand resulting in over-exploitation. Consequently, the plant is recognized as being "critically endangered" in its natural habitat. Development of an efficient Isolation, structure elucidation and biotransformation studies protocol will not only play a significant role in meeting the requirement of planting material for commercial cultivation, but also in aiding the conservation process.