Background: Urinary tract Infections (UTIs) are a cause of significant morbidity and mortality requiring urgent antibiotic treatment. However, there is widespread antibiotic-resistance from the bacterial causes, necessitating regular surveillance for drug-resistant bacteria and their antibiograms. Objective: The aim of this study is to isolate and identify various bacterial causes of UTIs and determine their antibiotic susceptibility patterns. Methods: A total of 5017 urine samples were analyzed from January 2021 to December 2022. Out of these 1014 samples were positive. Identification of the pathogen and antimicrobial sensitivity were done using the Automated system, Vitek 2 compact (Biomereiux). Results: Culture positivity in this study was 20%. Gram-negative bacteria were predominantly isolated (79.7%), including Escherichia coli (52.8%) and Klebsiella aerogenes (14.2%). Enterococcus faecalis was the most common Gram-positive bacterium isolated. Antibiotic susceptibility pattern among gram negative bacilli has shown maximum sensitivity to Colistin, Fosfomycin, followed by Carbapenems (Imipenem, Meropenem & Ertapenem), Amikacin and Beta lactam- betalactam inhibitor (BL-BLI) combination drugs. Out- patient department (OPD) samples were found to be more susceptible to antimicrobials than In-patient department (IPD) samples. Maximum resistance was seen against Ciprofloxacin and Ceftriaxone. Klebsiella and Pseudomonas isolates were found to be more resistant when compared to E.coli. E.coli isolates were more sensitive to Nitrofurantoin (87%) than Klebsiella (22%). Carbapenem resistance was seen in 50% of the Inpatient isolates of Klebsiella and 36% of Inpatient isolates of Pseudomonas.Few isolates of Klebsiella and Pseudomonas were found to be resistant even to Colistin

Cashew apple is a rich source of sugars and vitamins that is discarded as a waste during cashew nut processing. Cashew apples starts fermenting soon after the harvest and due to the presence of wide spectrum of polyphenols and tannins, change of colour of cashew apples lead to an unappealing marketability and storage stability. The present study is aimed to rapid identification of different spoilage yeasts and to identify them using MALDI-TOF Mass Spectroscopy. Spoilage yeasts like Candida krusei, C. tropicalis, Pichia norvegensis, Brettanomyces bruxellensis were the spoilage yeasts found to high confidence score values of 2.00 - 3.00 with high consistency