A series of 5-substituted (arylmethylene) pyridin-2-amine were synthesized by condensing various 5-substituted pyridyl-2-amines with various aromatic aldehydes. The structures of newly synthesized compounds were characterized by spectral and elemental analysis. All the compounds were screened for their antibacterial activities. The QSAR studies of were performed on MOE 2006.08 software. QSAR equation reveled that selected electronic, steric and liphophillic parameters have correlation with antibacterial activity. Best equations were selected on basis of correlation coefficient (r2) and predicitivity of equation. The frequent appearance of Log P and SMR terms in the QSAR equations is indicative of lipophilic and steric parameters are the prerequisites for molecules to exhibit activity against bacteria.

Scorpion venom is an antigenic, water-soluble, heterogenous mixture. The Na+-channel specific toxins from scorpions are modifiers of the channel gating mechanism. The recombinant DNA vaccines involved targeting multiple antigenic components to direct and empower the immune system to protect the host from infection. Limitation of therapy to the treatment of patients suffering from various adverse reaction and contraindications are always experienced. Antigenic epitope on iberiotoxin - eastern Indian scorpion are important determinant of protection against spider venom. As our knowledge of the immune responses to a protein antigen progressed, it became clear that the whole protein is not necessary for raising the immune response, but small segments (DVDCSVSKECWSVCKDLFG; 4-22) of protein called the antigenic determinant or the epitope is sufficient for eliciting the desired immune response. In analysis predicted antigenic epitope iberiotoxin protein is seen. Antigenic epitope from iberiotoxin is a predicted immunization strategy against various diseases.

The bee venom is used for treating a wide variety of disease conditions. It has also been found to be a strong immunological agent, stimulating the body's protective mechanisms against disease. The major treatment is gene therapy or recombinant DNA vaccines involved targeting multiple antigenic components to direct and empower the immune system to protect the host from infection. Limitation of therapy to the treatment of patients suffering from various adverse reaction and contraindications are always experienced. Tertiapin is a neurotoxin from the honeybee venom. It interacts specifically with calmodulin in the presence of Ca2+. Antigenic epitopes on Tertiapin protein of Apis mellifera (honey bee) is important determinants sites for protection against disorders. As our knowledge of the immune responses to a protein antigen progressed, it became clear that the whole protein is not necessary for raising the immune response, but small segments (NCNRIIIPHMCWK, 4-16) of tertiapin protein called the antigenic determinants or the epitopes are sufficient for eliciting the desired immune response. Immunization cassettes should be capable of immunizing of broad immunity against both humoral and cellular epitope thus giving vaccines the maximum ability to deal with Tertiapin protein of Apis mellifera immune escape. We have predicted a successful immunization strategy.

The toxin LTx5 toxin is an 116aa residue peptide is isolated from the venom of Lasiodora sp. IBSP8539.In this assay we have predicted the binding affinity of toxin LTx5 toxin having 116 amino acids, which shows 109 nonamers. Peptide fragments of the neurotoxin can be used to select nonamers for use in rational vaccine design and to increase the understanding of roles of the immune system in neurotoxin studies. Small segment ‘3- STFIIMISLAVALATWPSEH-22’ of toxin protein called the antigenic epitopes is sufficient for eliciting the desired immune response. Immunization cassettes should be capable of immunizing of broad immunity against both humoral and cellular epitope thus giving vaccines the maximum ability to deal with neurotoxin protein of Eurypelma californicum. Binding ability prediction of antigen peptides to MHC class molecules is important in vaccine development, We also found the SVM based MHCII-IAb peptide regions, 40- YALADRAEK, 10- ISLAVALAT, 99- MYQAERALE, 14- VALATWPSE, (optimal score is 1.494); MHCII-IAd peptide regions, 96- LDIMYQAER, 29- SETKLNVEL, 68- GASVLCEAV, 4- STFIIMISL, (optimal score is 0.604); MHCII-IAg7 peptide regions , 38- GPYALADRA, 98- IMYQAERAL, 9- MISLAVALA, 99- MYQAERALE, (optimal score is 1.904); and MHCII- RT1.B peptide regions, 17- ATWPSEHIE, 101- QAERALEKL, 98- IMYQAERAL, 108- KLASSFRCE, (optimal score is 0.601) which represented predicted binders from neurotoxin protein. We have predicted a successful immunization.