STUDY OF THE IL-23 RECEPTOR AND THE IL-28B GENE POLYMORPHISM IN INDIVIDUALS FROM THE LEPROSY-ENDEMIC AREA IN THE BRAZILIAN AMAZON

A.L.deS. GONCALVES¹, L.S. MOURA², M.E.S.de CARVALHO³, E.C.dos SANTOS⁴, M.doP.S.C.A. SILVESTRE⁵, A.B. SOUZA⁶, E.P.daC. GURRAO⁷, M.J.A. SILVA⁸*, K.V.B. LIMA⁹, L.N.G.C. LIMA^10
1Graduate Program in Parasitic Biology in the Amazon, University of Para State, Evandro Chagas Institute, Belem, Para, Brazil; Bacteriology and Mycology Section of the Evandro Chagas Institute, Belem, Para, Brazil
²Bacteriology and Mycology Section of the Evandro Chagas Institute, Belem, Para, Brazil
³Bacteriology and Mycology Section of the Evandro Chagas Institute, Belem, Para, Brazil
⁴Bacteriology and Mycology Section of the Evandro Chagas Institute, Belem, Para, Brazil
⁵Bacteriology and Mycology Section of the Evandro Chagas Institute, Belem, Para, Brazil
⁶Bacteriology and Mycology Section of the Evandro Chagas Institute, Belem, Para, Brazil
⁷Bacteriology and Mycology Section of the Evandro Chagas Institute, Belem, Para, Brazil
⁸Bacteriology and Mycology Section of the Evandro Chagas Institute, Belem, Para, Brazil
⁹Bacteriology and Mycology Section of the Evandro Chagas Institute, Belem, Para, Brazil
¹⁰Graduate Program in Parasitic Biology in the Amazon, University of Para State, Evandro Chagas Institute, Belem, Para, Brazil; Bacteriology and Mycology Section of the Evandro Chagas Institute, Belem, Para, Brazil
* Corresponding Author : jesseabrahao10@gmail.com

Received : 15-03-2022     Accepted : 26-05-2022     Published : 30-05-2022
Volume : 14     Issue : 1       Pages : 833 - 839
Genetics 14.1 (2022):833-839

Keywords : Cytokines, Leprosy, Immunogenetics, Single Nucleotide Polymorphism, Disease Susceptibility
Academic Editor : Prof Dr Suleyman Cylek, Dr Vipul N Kapadia
Conflict of Interest : None declared
Acknowledgements/Funding : Authors are thankful to Graduate Program in Parasitic Biology in the Amazon, University of Pará State, Evandro Chagas Institute, Belém, Pará, Brazil and Bacteriology and Mycology Section of the Evandro Chagas Institute, Belém, Pará, Brazil
Author Contribution : Conceptualization: L.N.G.C.L. and A.L.deS.G.; methodology: L.N.G.C.L., L.S.M. and M.E.dosS.C.; software; validation: K.V.B.L. and L.N.G.C.L.; formal analysis; investigation: L.S.M., M.E.S.deC., E.P.daC.G., A.L.deS.G. and M.doP.S.A.S.; resources: K.V.B.L.,

Leprosy is an immunopathology caused by Mycobacterium leprae and its clinical evolution depends on the immunological aspects of the host. There is evidence that IL28B acts by activating TNK cells and IL-23 promotes the expansion of Th17 cells, both actions are fundamental in the response to mycobacteria. We verified the relationship between the rs12979860 polymorphism in the IL28B gene and the rs11209026 polymorphism in the IL23 receptor gene and leprosy. 240 individuals from the Brazilian Amazon were included in the research, from which blood samples were collected for DNA extraction, SNP typing and sequencing. Individuals between 32 and 46 years old had 7.6 and individuals over 46 years old were 12.4 times more likely to become patients. No associations were observed between the rs12979860 SNP in the IL28B gene and the rs11209026 SNP in the IL23R, their genotypes and alleles and leprosy

1. Brazil (2017) Available: http://bvsms.saude.gov.br /bvs/publicacoes/guia_pratico_ hanseniase.%0Apdf
2. de Sousa J.R., Sotto M.N., Simões Quaresma J.Á. (2017) Front Immunol., 8, 1635-1635. doi:10.3389/fimmu.2017.01635
3. Silva J.S.R. da, Palmeira I.P., Sá AMM, Nogueira L.M.V., Ferreira A.M.R., Silva JSR da, et al. (2018) Revista Cuidarte., 9, 2338-2348. doi:10.15649/cuidarte.v9i3.548
4. Goulart I.M.B., Penna G.O., Cunha G. (2002) Rev Soc Bras Med Trop., 35, 363-375. doi:10.1590/S0037-86822002000400014
5. Mendonça V.A., Costa R.D., Melo GEBA de, Antunes C.M., Teixeira A.L. (2008) An Bras Dermatol., 83, 343-350. doi:10.1590/S0365-05962008000400010
6. Sadhu S., Mitra D.K. Emerging (2018) Front Immunol., 9, 604-604. doi:10.3389/fimmu.2018.00604
7. Schreuder P.A.M., Noto S., Richardus J.H. (2016) Clinics in Dermatology., 34, 24-31. doi:10.1016/j.clindermatol.2015.11.001
8. dos Reis A.S. (2018) Revista de Ciências da Saúde., 20, 33-40.
9. Sadhu S., Khaitan B.K., Joshi B., Sengupta U., Nautiyal A.K., Mitra D.K. (2016) PLoS Negl Trop Dis., 10, e0004338-e0004338. doi:10.1371/journal.pntd.0004338
10. Pinheiro R.O., Schmitz V., Silva B.J. de A., Dias A.A., de Souza B.J., de Mattos Barbosa M.G., et al. (2018) Front Immunol., 9, 518-518. doi:10.3389/fimmu.2018.00518
11. Santos S.M.F. dos, Sousa M.T. de, Santos L.A. dos, Jacob LM da S, Figueira MC e S, De Melo MC. (2018) J Health Scie., 20, 61. doi:10.17921/2447-8938.2018v20n1p61-67
12. de Sousa C.R.S., Feitosa M.C. da R, Pinheiro A.B.F., Cavalcante K.K. de S. (2019) RBPS., 32, 1-10. doi:10.5020/18061230.2019.9469
13. Rodrigues A.G. (2008), 107.
14. Rezende D.V.P. (2013) Available: http://www.repositorio.ufc.br/handle/riufc/6892
15. Saini C., Ramesh V., Nath I. (2014) PLoS Negl Trop Dis., 8, e2639-e2639. doi:10.1371/journal.pntd.0002639
16. Passos I. da M. (2011) Universidade Federal do Amazonas. Available: https://tede.ufam.edu.br/handle/tede/5197
17. Bravo D., Solano C., Giménez E., Remigia M.J., Corrales I., Amat P., et al. (2014) J Med Virol., 86, 838-844. doi:10.1002/jmv.23865
18. El-Garawani I., Hassab El-Nabi S., Gadallah M., Abdelsameea E. (2021) Immunol Invest., 50, 12-22. doi:10.1080/08820139.2020.1722158
19. Falleti E., Bitetto D., Fabris C., Cussigh A., Fornasiere E., Cmet S., et al. (2011) J Clin Immunol., 31, 891-899. doi:10.1007/s10875-011-9547-1
20. Li G.D., Wang D., Zhang D.F., Xiang Q., Feng J.Q., Li X.A., et al. (2016) J Dermatol Sci., 84, 322-329. doi:10.1016/j.jdermsci.2016.09.018
21. Machmach K., Abad-Molina C., Romero-Sánchez M.C., Abad M.A., Ferrando-Martínez S., Genebat M., et al. (2013) J Infect Dis., 207, 651-655. doi:10.1093/infdis/jis717
22. Manry J., Quintana-Murci L. (2013) Cold Spring Harbor perspectives in medicine., 3, a012450.
23. Moura K.C.R. de (2015) Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Medicina de Botucatu. Available: https://repositorio.unesp.br/handle/11449/126466
24. Oliveira N.C. de, Pfrimer I.A.H. (2019) Revista Brasileira de Análises Clínicas., 51. doi:10.21877/2448-3877.201900708
25. Wu Y., Lu Z., Chen Y., Xue F., Chen X., Zheng J. (2010) Chinese Journal of Dermatology., 43, 773-776.
26. Zhang F., Liu H., Chen S., Low H., Sun L., Cui Y., et al. (2011) Nat Genet., 43, 1247-1251. doi:10.1038/ng.973
27. Zhang Y., Zhu S.L., Chen J., Li L.Q. (2016) Onco Targets Ther., 9, 3249-3257. doi:10.2147/OTT.S104904
28. Dallmann-Sauer M., Correa-Macedo W., Schurr E. (2018) Mamm Genome., 29, 523-538. doi:10.1007/s00335-018-9765-4
29. Ge D., Fellay J., Thompson A.J., Simon J.S., Shianna K.V., Urban T.J., et al. (2009) Nature., 461, 399-401. doi:10.1038/nature08309
30. Grebely J., Petoumenos K., Hellard M., Matthews G., Suppiah V., Applegate T., et al. (2010) Hepatology., 52, 1216-1224. doi:10.1002/hep.23850
31. Lin C.Y., Chen J.Y., Lin T.N., Jeng W.J., Huang C.H., Huang C.W., et al. (2011) PLoS One., 6, e18322-e18322.
32. Mehta M., Hetta H.F., Abdel-Hameed E.A., Rouster S.D., Hossain M., Mekky M.A., et al. (2016) Arch Virol., 161, 3161-3169.
33. Morrow M.P., Yan J., Pankhong P., Shedlock D.J., Lewis M.G., Talbott K., et al. (2010) Mol Ther., 18, 1714-1723. doi:10.1038/mt.2010.118
34. Li Y., Yu X., Ma Y., Hua S. (2019) Cytokine., 120, 78-84.
35. Amaral I. do S.A. (2015) Universidade Federal do Pará. 2015. Available: http://repositorio.ufpa.br:8080/jspui/handle/2011/9107
36. Ramos N.M.D. (2015) Thesis. Available: https://www.arca.fiocruz.br/handle/icict/13628
37. Abdollahi E., Tavasolian F., Momtazi-Borojeni A.A., Samadi M, Rafatpanah H. (2016) J Immunotoxicol., 13, 286–300. doi:10.3109/1547691X.2015.1115448
38. Esberard B. (2012) Revista Hospital Universitário Pedro Ernesto., 11. Available: https://www.e-publicacoes.uerj.br/index.php/revistahupe/article/view/9000
39. Duerr R.H., Taylor K.D., Brant S.R., Rioux J.D., Silverberg M.S., Daly M.J., et al. (2006) Science., 314, 1461–1463. doi:10.1126/science.1135245
40. Ben-Selma W., Boukadida J. (2012) Clin Vaccine Immunol., 19, 1188–1192. doi:10.1128/CVI.00135-12
41. Brown M.A. (2009) Arthritis Research & Therapy., 11, 1–6.
42. Nair R.P., Ruether A., Stuart P.E., Jenisch S., Tejasvi T., Hiremagalore R., et al. (2008) J Invest Dermatol., 128, 1653-1661. doi:10.1038/sj.jid.5701255
43. Weersma R.K., Stokkers P.C.F., van Bodegraven A.A., van Hogezand R.A., Verspaget H.W., de Jong D.J., et al. (2009) Gut., 58, 388-395. doi:10.1136/gut.2007.144865
44. Reis A. da S. dos, Souza E.A. de, Ferreira A.F., Silva G.V. da, Macedo S.F. de, Araújo O.D. de, et al. (2019) Cad Saúde Pública., 35. doi:10.1590/0102-311X00014419
45. Barbosa J.C., Ramos Junior A.N., Alencar O.M., Pinto M.S.P., Castro C.G.J. de. (2014) Cad saúde colet., 22, 351-358. doi:10.1590/1414-462X201400040008
46. Monteiro M.J. de S.D., Santos G.M. dos, Barreto M.T.S., Silva R.V. de S., Jesus R.L.R. de, Silva H.J.N. da. (2017) Revista de Atenção à Saúde., 15, 21-28. doi:10.13037/ras.vol15n54.4766
47. Pimentel C. da R., Colacite J. (2021) Brazilian Journal of Health Review., 4, 3549-3559. doi:10.34119/bjhrv4n1-277
48. Oliveira A.E.V.M. de, Araújo K.M. da F.A., Queiroga R.P.F. de, Bezerra L.L.O., Chaves A.E.P. (2020) Research, Society and Development., 9, e755985778-e755985778. doi:10.33448/rsd-v9i8.5778
49. Silva L.S.R. da, Miritiba C.S., Silva K.J. de S. da, Pessoa I.M., Silva A.A., Jardim M.J.A., et al. (2020) Acervo Saúde., 12, e3902. doi:10.25248/reas.e3902.2020
50. Silva P.L.N. da, Silva E.L.G., Santos V.M., Galvão A.P.F.C., Oliveira V.V. de, Alves C. dos R. (2021) Nursing, 24, 5377-5388. doi:10.36489/nursing.2021v24i274p5377-5388
51. Sarin R., Wu X., Abraham C. (2011) Proc Natl Acad Sci U S A., 108, 9560-9565. doi:10.1073/pnas.1017854108
52. Sayed M.I., Hashad D.I., Soliman E.A., Talaaba M.M. (2018) Alexandria Journal of Medicine., 54, 299-302. doi:10.1016/j.ajme.2017.08.005
53. Jiang D., Wubuli A., Hu X., Ikramullah S., Maimaiti A., Zhang W., et al. (2015) BMC Infectious Diseases., 15, 550. doi:10.1186/s12879-015-1284-2
54. Leturiondo A.L., Noronha A.B., Mendonça C.Y.R., Ferreira C. de O, Alvarado-Arnez L.E., Manta F.S. de N., et al. (2020) PLoS Negl Trop Dis., 14, e0008247-e0008247. doi:10.1371/journal.pntd.0008247
55. Deveci H., Turk A.C., Ozmen Z.C., Demir A.K., Say Coskun S.U. (2019) Cent Eur J Immunol., 44, 433-439. doi:10.5114/ceji.2019.92805
56. Xia Y., Liang Y., Guo S., Yu J.G., Tang M.S., Xu P.H., et al. (2018) Postgrad Med J., 94, 508-516. doi:10.1136/postgradmedj-2018-135665
57. Filiz B., Y?ld?r?m M., Hekimler Öztürk K., ?irin F.B., Çelik S., Erturan ?., et al. (2019) Turk J Med Sci., 49, 1386-1394. doi:10.3906/sag-1904-48
58. Loures M.A.R., Alves H.V., de Moraes A.G., Santos T. da S., Lara F.F., Neves J.S.F., et al. (2019) Expert Rev Clin Immunol., 15, 303-313. doi:10.1080/1744666X.2019.1564039
59. Hamdy G., Darweesh H., Khattab E.A., Fawzy S., Fawzy E., Sheta M. (2015) Hum Immunol., 76, 417-420. doi:10.1016/j.humimm.2015.03.020
60. Zou Q., Zhao Y., Wang Y., Fang Y., Liu Y. (2019) Artificial Cells, Nanomedicine, and Biotechnology., 47, 951-956. doi:10.1080/21691401.2019.1579731
61. Paradowska-Gorycka A., Malinowski D., Haladyj E., Olesinska M., Safranow K., Pawlik A. (2018) Med Clin (Barc), 151, 191-195. doi:10.1016/j.medcli.2017.11.029
62. Li W., Shi W., Yin Y., Chen J., Luo L. (2020) Pharmgenomics Pers Med., 13, 725-733. doi:10.2147/PGPM.S275073
63. Zhu Y., Jiang H., Chen Z., Lu B., Li J., Shen X. (2020) Inflammation Research., 69, 87-103.
64. Li Y., Mao Q., Shen L., Tian Y., Yu C., Zhu W.M., et al. (2010) Inflamm Res., 59, 607-614. doi:10.1007/s00011-010-0171-y
65. Nossent J.C., Sagen-Johnsen S., Bakland G. (2018) Rheumatol Adv Pract. 2, rky006-rky006. doi:10.1093/rap/rky006
66. Zhao L., Zhu H., Han B., Wang L., Sun Y., Lu X., et al. (2018) Ann Hematol., 97, 685-695. doi:10.1007/s00277-018-3227-7
67. Pineda J.A., Caruz A., Rivero A., Neukam K., Salas I., Camacho A., et al. (2010) Clin Infect Dis., 51, 788-795. doi:10.1086/656235
68. Lu Y.F., Goldstein D.B., Urban T.J., Bradrick S.S. (2015) Virology., 476, 334-340. doi:10.1016/j.virol.2014.12.020
69. Muir A.J. (2014) Am J Gastroenterol., 109, 628-635. doi:10.1038/ajg.2014.66
70. Angulo J., Pino K., Echeverría-Chagas N., Marco C., Martínez-Valdebenito C., Galeno H., et al. (2015) Clin Infect Dis., 61, e62-e69. doi:10.1093/cid/civ830
71. Attallah A.M., Omran D., Marie M.S., Abdelrazek M., Salama A., El Essawey R, et al. (2018) Br J Biomed Sci., 75, 157-162. doi:10.1080/09674845.2018.1489599
72. Machicote A., Flichmann D., Arana E., Paz S., Fainboim H., Fainboim L., et al. (2018) International Journal of Clinical Medicine., 9, 79-91. doi:10.4236/ijcm.2018.92009
73. Vargas-Castillo A.B., Ruiz-Tovar K., Vivanco-Cid H., Quiroz-Cruz S., Escobar-Gutiérrez A., Cerna-Cortes J.F., et al. (2018) Viral Immunol., 31, 249-255. doi:10.1089/vim.2017.0069
74. Ouladlahsen A., Bensghir R., Baba H., Haddaji A., Abbadi I., Zaidane I., et al. (2020) Pathobiology., 87, 262-267. doi:10.1159/000507763
75. Zaidane I., Wakrim L., Oulad Lahsen A., Bensghir R., Chihab H., Jadid F.Z., et al. (2018) Hum Immunol., 79, 70-75. doi:10.1016/j.humimm.2017.10.011
76. Czech-Kowalska J., Jedli?ska-Pijanowska D., Pleskaczy?ska A.K., Niezgoda A., Gradowska K., Pietrzyk A., et al. (2021) Viruses., 13, 1783. doi:10.3390/v13091783
77. Jedli?ska-Pijanowska D., Kasztelewicz B., Czech-Kowalska J., Jaworski M., Charusta-Sienkiewicz K., Dobrza?ska A. (2020) PLoS One., 15, e0233096. doi:10.1371/journal.pone.0233096
78. Chmelova K., Frankova S., Jirsa M., Neroldova M., Sticova E., Merta D., et al. (2019) Transpl Infect Dis., 21, e13124. doi:10.1111/tid.13124
79. Vallinoto A.C.R., Santana B.B., Sá K.S.G., Ferreira T.C.S., Sousa R.C.M., Azevedo V.N., et al. (2015) Mediators Inflamm., 2015, 804167-804167. doi:10.1155/2015/804167
80. de Sá K.S.G., Santana B.B., de Souza Ferreira T.C., Sousa R.C.M., Caldas C.A.M., Azevedo V.N., et al. (2016) Cytokine., 77, 79-87. doi:10.1016/j.cyto.2015.11.004
81. Pérez-García F., Jiménez-Sousa M.Á., Soria S., Jorge-Monjas P., Fernández-Rodríguez A., Gómez-Sánchez E., et al. (2020) Front Med (Lausanne), 7, 186-186. doi:10.3389/fmed.2020.00186