PRODUCTION OF BIOSURFACTANT FROM DATES AND ITS ACTIVITY AGAINST BIOFILM PRODUCED BY CANDIDA AND E. COLI

H.A. PATHAN¹*, S. SHAIKH², S. KHAN³, S. SHARMA⁴, P. PAL⁵, S.D. WAVHAL^6
1Department of Biotechnology, Ismail Yusuf College, Mumbai, 400060, University of Mumbai, Mumbai, Maharashtra, India
²Department of Biotechnology, Ismail Yusuf College, Mumbai, 400060, University of Mumbai, Mumbai, Maharashtra, India
³Department of Biotechnology, Ismail Yusuf College, Mumbai, 400060, University of Mumbai, Mumbai, Maharashtra, India
⁴Department of Biotechnology, Ismail Yusuf College, Mumbai, 400060, University of Mumbai, Mumbai, Maharashtra, India
⁵Department of Biotechnology, Ismail Yusuf College, Mumbai, 400060, University of Mumbai, Mumbai, Maharashtra, India
⁶Department of Biotechnology, Ismail Yusuf College, Mumbai, 400060, University of Mumbai, Mumbai, Maharashtra, India
* Corresponding Author : hinaa_123@yahoo.com

Received : 01-10-2020     Accepted : 23-10-2020     Published : 30-10-2020
Volume : 12     Issue : 10       Pages : 1917 - 1921
Int J Microbiol Res 12.10 (2020):1917-1921

Keywords : Biosurfactant, Dates, Biofilm
Academic Editor : Rajpal Diwakar
Conflict of Interest : None declared
Acknowledgements/Funding : Authors are thankful to Department of Biotechnology, Ismail Yusuf College, Mumbai, 400060, University of Mumbai, Mumbai, Maharashtra, India
Author Contribution : All authors equally contributed

The aim of this research was production of biosurfactant from the normal flora of Dates (Phoenix dactylifera) to check its activity against biofilm produced by Candida and E.coli. The organisms were isolated LBD (Lactic acid bacteria differential agar) and the plates were incubated at room temperature for 48 hours. Several biochemical test were performed with this strains. Drop collapse test was performed and was found to be +ve. The organism was inoculated in MSM broth (mineral salt medium) with petrol as a source of carbon for biosurfactant production and organism was found to be degraded in MSM broth. Biosurfactant was extracted and purified in ethyl acetate and methanol (4:1). The biosurfactant extracted was checked against biofilm formed by Candida in catheter and E.coli in IV set and was incubated overnight. After incubation catheter and IV set was washed 3 times with saline. For checking the biosurfactant activity Catheter and IV set was dipped in saline with biosurfactant by cutting them into small pieces. They were incubated overnight and Spread plate was performed for each wash and variation in growth was observed in gradient form. The organism was characterised at NFB by 16s RNA sequencing method and was found to be Acinetobacter pittii