ISOLATION, IDENTIFICATION AND CHARACTERIZATION OF PASTEURELLA MULTOCIDA ISOLATED FROM PIGS OF MIZORAM, MANIPUR AND ASSAM

S. MUKHERJEE¹*, L. BABITA DEVI², G. MAHATO³, R.K. SHARMA^4
1College of Veterinary Sciences & Animal Husbandry, Central Agricultural University, Jalukie, 797110, Nagaland, India
²ICAR-Krishi Vigyan Kendra, Peren, ICAR Nagaland Centre, Jalukie, Peren, 797110, Nagaland, India
³College of Veterinary Sciences, Assam Agricultural University, Khanapara, Guwahati, 781022, Assam, India
⁴College of Veterinary Sciences, Assam Agricultural University, Khanapara, Guwahati, 781022, Assam, India
* Corresponding Author : shyamananda1@rediffmail.com

Received : 01-07-2020     Accepted : 13-07-2020     Published : 30-07-2020
Volume : 12     Issue : 7       Pages : 1890 - 1892
Int J Microbiol Res 12.7 (2020):1890-1892

Keywords : Pasteurella multocida, Pig, pm-PCR, Cap-PCR
Academic Editor : Naserzadeh Yousef, Dr C. Lalmuanthanga, Dr H. V. Pandya, Kalaivanan D., Divyanshu Gupta, Dey S., Rajpal Diwakar, Dr G. P. Kalmath, Dr Jaysankar De, Janmajoy Banerjee
Conflict of Interest : None declared
Acknowledgements/Funding : Authors are thankful to Central Agricultural University, Imphal; Department of Veterinary Epidemiology & Preventive Medicine, Department of Microbiology, Department of Clinical Medicine and Department of LPM, College of Veterinary Science Assam Agricultural University (AAU), Khanapara for providing the required instrumentation facilities. The authors are also thankful to College of Veterinary Sciences, Assam Agricultural University, Khanapara, Guwahati, 781022, Assam, India for providing infrastructure facilities to conduct the research work.
Author Contribution : All authors equally contributed

Total 171 trachea/ nasal swab and lungs samples of pigs collected from Mizoram (40 samples), Manipur(51 samples) and Assam (80 samples), 17 (9.94 %) were found to be positive for P. multocida. Fourteen of the 17 isolates subjected to PCR targeting species-specific KMT 1 gene revealed a distinct band of 460 bp, which was a confirmation of the isolates as P. multocida. Among the confirmed P. multocida isolates, nine were identified as capsular type A (1044 bp), while the remaining five were capsular type D (657 bp), based on multiplex cap-PCR results, targeting hyaD-hyaC and dcbF genes, respectively.

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