ANTIMICROBIAL ACTIVITIES OF Pleurotus SPECIES AGAINST HUMAN PATHOGENIC BACTERIA

R.S. JARIAL¹*, A. SHARMA², K. JARIAL³, S. JANDAIK^4
1College of Horticulture and Forestry, Neri, Hamirpur, 177001, Dr YS Parmar University of Horticulture and Forestry, Nauni, 173230, Himachal Pradesh, India
²College of Horticulture and Forestry, Neri, Hamirpur, 177001, Dr YS Parmar University of Horticulture and Forestry, Nauni, 173230, Himachal Pradesh, India
³College of Horticulture and Forestry, Neri, Hamirpur, 177001, Dr YS Parmar University of Horticulture and Forestry, Nauni, 173230, Himachal Pradesh, India
⁴College of Horticulture, Dr YS Parmar University of Horticulture and Forestry, Nauni, 173230, Himachal Pradesh, India
* Corresponding Author : rsjarial@rediffmail.com

Received : 02-05-2020     Accepted : 20-05-2020     Published : 30-05-2020
Volume : 12     Issue : 5       Pages : 1843 - 1847
Int J Microbiol Res 12.5 (2020):1843-1847

Keywords : Antibacterial property, Bacillus sp. E. coli, Pleurotus florida, Pleurotus ostreatus, Pleurotus sajor-caju, , Proteus sp., Pseudomonas sp., Streptococcus sp
Academic Editor : Takemi Otsuki, Tarek Mohamed Abdel Ghany, Dr Sabyasachi Chatterjee
Conflict of Interest : None declared
Acknowledgements/Funding : Authors are thankful to College of Horticulture and Forestry, Neri, Hamirpur, 177001, Dr YS Parmar University of Horticulture and Forestry, Nauni, 173230, Himachal Pradesh, India
Author Contribution : All authors equally contributed

The fruiting bodies and mycelium of mushrooms exhibit health promoting values including antibacterial activities. Among different cultivated mushrooms, Pleurotus species are easy to cultivate and also exhibit many medicinal properties. Many of the Pleurotus species have also been reported to exhibit antimicrobial action against different pathogens associated with human beings. Keeping this in view, antimicrobial activities of three species of Pleurotus viz., P. florida, P. ostreatus and P. sajor-caju were screened by paper disc method against human pathogenic bacteria viz., Escherichia coli, Pseudomonas sp., Proteus sp., Bacillus sp. and Streptococcus sp. by preparing the aqueous, hot water, ethanolic and methanolic extracts of mycelia, pileus and stipe. Among aqueous extracts, stipe extracts of all the three species were effective in inhibiting the growth of human pathogenic bacteria (9.33 mm), P. sajor-caju being the most effective in terms of inhibiting all the five test bacteria. However, hot water, ethanolic and methanolic extracts of pileus of three Pleurotus spp. were found to be most effective in terms of exhibiting maximum zone of inhibition (8.16, 6.64 and 7.23 mm, respectively). Among the three species, hot water extract of P. ostreatus (3.20 mm) and ethanolic as well as methanolic extracts of P. sajor-caju (2.09 and 4.02 mm, respectively) were most effective against the test bacteria.

1. Vamanu E., Pelinescu D., Avram I. (2018) Polish Journal of Food and Nutrition Sciences, 68 (1), 83-90.
2. Elisashvili V. (2012) International Journal of Medicinal Mushrooms, 14 (3), 211-239.
3. Golak-Siwulska I., Ka?uzewicz A., Spi zewski T., Siwulski M., Sobieralski K. (2018) Folia Horticulturae, 30 (2), 191-201.
4. Chang S.T., Miles P.G. (1989) Edible mushrooms and their cultivation. CRC press, Baco Raton. FL.
5. Kues U., Liu Y. (2000) Applied Microbiology and Biotechnology, 54, 141-152.
6. Upadhyay R.C. (2011) In: Mushrooms: Cultivation Marketing and Consumption (Manjit Singh, B Vijay, Shwet Kamal and GC Wakchaureeds). Directorate of Mushroom Research (ICAR) Chambaghat Solan HP, pp 129-138.
7. Randive S.D. (2012) Advances in Applied Science Research, 3(4), 1938-1949.
8. Pandiarajan G., Govindaraj R., Jeyaraman M., Kumar B.M. (2012) International Journal of Pharmacy and Pharmaceutical Sciences, 4(2), 238-240.
9. Ahmed M.Y., Fang-Sheng W., Hussien H.E.S. (2015) International Journal of Medicinal Mushroom, 17 (6), 579-590.
10. Owaid M.N., Sajid S.S., Al-Saeedi, Idham Abed Ali Al-Assaffii (2015) Journal of Medicinal and Bioengineering, 4(5), 376-380.
11. Vamanu E. (2012) Molecules, 17, 3653-3671.
12. Dogan H.H., Duman R., Ozkalp B., Aydin S. (2013) Pharmaceutical Biology, 51, 707-711.
13. Heleno S.A., Stojkovic D., Barros L., Glamoclija J., Sokovic M., Martins A., Queiroz M.J.R.P., Ferreira I.C.F.R. (2013) Food Research International, 51, 236-243.
14. Khan M.A., Rahman M.M., Tania M., Uddin N., Ahmed S. (2011) The Open Nutraceuticals Journal, 4, 20-24.
15. Nithya R., Ragunathan R. (2009) Digest Journal Nanomaterials and Biostructures, 4(4), 623-629.
16. Thillaimaharani K.A., Sharmila K., Thangaraju P., Karthick M., Kalaiselvam M. (2012) International Journal of Pharmaceutical Sciences and Research, 4(4), 1540-1545.
17. Sathyan A., Khadeeja A.M., Majitha V.K., Rajeswary K.R. (2017) International Journal of Agricultural Innovations and Research, 5 (6), 907- 912.