ISSN : 0975-5276
EISSN : 0975-9174
H. DAS1, G. KAUR2*, M. CHANDRA3, P.N. DWIVEDI4
1Department of Veterinary Microbiology, COVS, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, 141001, Punjab, India
2Department of Veterinary Microbiology, COVS, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, 141001, Punjab, India
3Department of Veterinary Microbiology, COVS, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, 141001, Punjab, India
4Department of Veterinary Microbiology, COVS, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, 141001, Punjab, India
* Corresponding Author : gurpreet7502@rediffmail.com
Received : 18-11-2019 Accepted : 26-11-2019 Published : 30-11-2019
Volume : 11 Issue : 11 Pages : 1739 - 1741
Int J Microbiol Res 11.11 (2019):1739-1741
Keywords : Canine Parvovirus, dogs, PCR, Nested PCR
Academic Editor : Sunil Sonu Hatkar, Dr Dibyajyoti Talukda, Lalit Batra, Dr Abhijit K Barate
Conflict of Interest : None declared
Acknowledgements/Funding : Authors are thankful to DBT for funding the research project (BT/ADV/Canine Health/TANUVAS/2017-18) and to the Director of research, Guru Angad Dev Veterinary and Animal Sciences University for providing the research facilities. The authors are also thankful for the Director TVCC Khanapara, C.V.Sc. AAU for providing samples for the research
Author Contribution : All authors equally contributed
Canine parvovirus a single stranded DNA virus causes hemorrhagic gastroenteritis in dogs and is the major cause of mortality in pups due to gastroenteritis. The virus has three major antigenic types CPV 2a, CPV 2b and CPV 2c which are prevalent in dog population worldwide. The virus is excreted heavily in feces of affected dogs, so can be easily detected in the feces of the affected dogs. Therefore, in the present study fecal samples were collected from the dogs suspected of CPV, DNA extracted from them and subjected to PCR and nested PCR to study the prevalence of CPV in two regions viz. Punjab and Assam regions of India. The prevalence of CPV was found to be 75% in Punjab and 80.55% in Assam by nested PCR with overall prevalence being 77%. Positivity for CPV was also observed in dogs which were vaccinated for the disease with percent positivity in Punjab being 84.6% and in Assam being 72.7% by nested PCR. The overall percent positivity in vaccinated dogs was found to be 79.16%. Therefore, it can be concluded that CPV is highly prevalent in the regions under study.
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