SCREENING AND CHARACTERIZATION OF L-ASPARAGINASE PRODUCING BACTERIA FROM SOIL SAMPLE

P. SHRIVASTAVA¹, M.K. YADAV²*
¹Department of Plant Pathology, S.S. Memorial Mahavidyalaya, Takha, Etawah, 206242, Chhatrapati Shahu Ji Maharaj University, Kanpur, 208024, Uttar Pradesh, India
²Department of Plant Pathology, Janta College, Bakewar, Etawah, 206124, Chhatrapati Shahu Ji Maharaj University, Kanpur, 208024, Uttar Pradesh, India, India
* Corresponding Author : manojbhu87@gmail.com

Received : 01-11-2019     Accepted : 26-11-2019     Published : 30-11-2019
Volume : 11     Issue : 11       Pages : 1727 - 1729
Int J Microbiol Res 11.11 (2019):1727-1729

Keywords : De- novo, Erwinia carotovora, L-Asparaginase, Lymphoblastic leukemia
Academic Editor : Kiranjot Kaur, Mahajan V., Ravindra Raosaheb Rakh, Kanagasanthosh K.
Conflict of Interest : None declared
Acknowledgements/Funding : Authors are thankful to Department of Plant Pathology, S.S. Memorial Mahavidyalaya, Takha, Etawah, 206242, Chhatrapati Shahu Ji Maharaj University, Kanpur, 208024, Uttar Pradesh, India. Authors are also thankful to Department of Microbiology, College of Life Science, CHRI Campus, Gwalior, M.P.
Author Contribution : All authors equally contributed

Asparaginase isolated from muddy soil, collected from cancer hospital garden and some other places of Gwalior. L-Asparaginases have been used as an anti-tumor agent for the effective treatment of acute lymphoblastic leukemia and food processing and reduce the acrylamide formation during frying of starchy food at high temperature. In the present study, we isolate bacteria (producing asparaginase enzyme) strains from soil. The isolated bacteria were screened for L-Asparaginase producing using M-9 medium on the basis of pink zone formation. An enzyme isolated from the bacterium Escherichia coli or Erwinia carotovora with anti-leukemic activity. L-Asparaginase are hydrolyzed into L-aspartic acid and ammonia in leukemic cells, resulting in the depletion of asparagine, inhibition of protein synthesis, cell cycle arrest in the G1 phase, and apoptosis in susceptible leukemic cell populations. The Erwinia carotovora -derived form of asparaginase is typically reserved for case. At present, the principal source of L-asparaginase for clinical trials is the bacteria E. coli; several other alternative sources are screen for production of large quantities of L-asparaginase then E. coli.

1. Broome J. (1961) Nature, (171), 1114.
2. Curran M., Daniel R., Guy G. and Morgan H. (1985) Arch. Biochem. Biophys., (241), 571.
3. Mashburn L. and Wriston J. (1963) Biochem. Biophys. Res. Commun., (12), 50.
4. Lauinger C. and Ressler C. (1970) Biochim. Biophys. Acta, (198), 316.
5. Kristiansen T., Einarsson M., Sunderberg L. and Porath J. (1970) FEBS Lett., (7), 294.
6. Kamble K.D., Bidwe P.R., Muley V.Y., Kamble L.H., Bhadange D.G. and Musaddiq M. (2012) Bioscience Discovery, 3(1),116-119.
7. Agarwal A., Kumar S. and Veeranki V. (2011) Letters in Applied Microbiology, (10), 1111.
8. Osama M. Darwesh, Mohamed F. Eida and Ibrahim A. Matter (2018) Bioscience Research, 15(3), 2802-2812.