GREEN BEAN COFFEE AS NUTRIENT SOURCE FOR INSECTICIDE DEGRADING BACTERIA

P.D. YADAV¹, S. KADAM², H.A. PATHAN³*, S.D. WAVHAL^4
1Ismail Yusuf College, Mumbai, 400060, University of Mumbai, Mumbai, Maharashtra, India
²Ismail Yusuf College, Mumbai, 400060, University of Mumbai, Mumbai, Maharashtra, India
³Ismail Yusuf College, Mumbai, 400060, University of Mumbai, Mumbai, Maharashtra, India
⁴Ismail Yusuf College, Mumbai, 400060, University of Mumbai, Mumbai, Maharashtra, India
* Corresponding Author : hinaa_123@yahoo.com

Received : 07-04-2019     Accepted : 26-06-2019     Published : 30-06-2019
Volume : 11     Issue : 6       Pages : 1624 - 1627
Int J Microbiol Res 11.6 (2019):1624-1627

Keywords : Coffee, Insecticide, Biodegradation
Academic Editor : Amit Fulzele
Conflict of Interest : None declared
Acknowledgements/Funding : Authors are thankful to Ismail Yusuf College, Mumbai, 400060, University of Mumbai, Mumbai, Maharashtra, India
Author Contribution : All authors equally contributed

The main criteria of this research were to isolate organism from green coffee bean which can degrade insecticide (chloropyrifos). Coffee bean crushed and inoculated in MSM medium incubated at static condition on shaker for 1 week then transfer to fresh MSM medium of one-week incubation each. Then the organism was isolated on MSM agar medium by T plate method & spread plate method. Then the organism was characterized by biochemical test. This organism was characterized by CSIR-NCL Pune for 16s RNA sequencing. The organism was found to resemble (Enterobacter chuandaensis) by 16s RNA. This organism was exposed to different concentration of chlorpyrifos(2%,1%,0.5%,0.3%,0.2%) and spectrophotometer analysis was carried out and best result of degradation was obtained in 0.5%. result showed that coffee bean was an adequate nutrient source for bacterial growth and it significantly enhanced chlorpyrifos biodegradation.

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