PREVALENCE OF POTENTIAL PATHOGENIC AEROMONADS SPECIES IN SEA FISH, CRAB AND PRAWNS IN CHENNAI, INDIA

RONGSENSUSANG¹*, S.J. DEEPAK², C.O. VINAYANANDA³, A. ELANGO⁴, K. PORTEEN^5
1Department of Livestock Products Technology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai, 600051 India
²Department of Veterinary Public Health and Epidemiology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai, 600051 India
³Department of Livestock Products Technology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai, 600051 India
⁴Department of Veterinary Public Health and Epidemiology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai, 600051 India
⁵Department of Veterinary Public Health and Epidemiology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai, 600051 India
* Corresponding Author : rongsen77@gmail.com

Received : 01-11-2018     Accepted : 27-11-2018     Published : 30-11-2018
Volume : 10     Issue : 11       Pages : 1414 - 1417
Int J Microbiol Res 10.11 (2018):1414-1417

Keywords : Aeromonas spp., Antimicrobial resistance, Cross contamination, Potential pathogenic, Public Health crisis
Conflict of Interest : None declared
Acknowledgements/Funding : Authors are thankful to Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai, 600051, Tamil Nadu, India
Author Contribution : All author equally contributed

The study was undertaken to assess the prevalence of Aeromonads and their antimicrobial resistance and virulence genes potentials among isolated Aeromonas species from seafood such as fish, crab and prawns in Chennai, India. Total of 270 fresh sea foods samples - 30 samples of each sea foods viz., sea fish, prawn and crabs from each of 3 fish markets i.e., Marina beach, Chintadripet and Purasaiwakkam. Among 270 samples investigated, 172 samples showed positive by cultural method. The duplex PCR was performed to detect the 16S rRNA and aerolysin (aerA) gene in the isolates. Out of which, 160/172 samples were confirmed as Aeromonas species targeting 16S rRNA and 57/160 (35.53%) showed positive for aerolysin gene, a pathogenic marker. The antimicrobial resistance pattern of Aeromonas spp. isolates showed complete resistant against ampicillin, penicillin and cephalothin whereas sensitive against enrofloxacillin, gentamicin and tetracycline. The increased prevalence of aeromonads in sea food is attributed to cross contamination during post-harvest processing. The detection of potential pathogenic aeromonads and multiple antimicrobial resistances in Aeromonas species isolated raise serious public health concern worthy of further investigation.

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