IN VITRO EVALUATION OF DIFFERENT MEDIA AGAINST GROWTH AND SPORULATION OF PYRICULARIA GRISEA ISOLATES CAUSING RICE BLAST

CH VARAPRASADA RAO¹*, P. ANIL KUMAR^2
1ICAR-Krishi Vigyan Kendra, Darsi, Prakasam, 523247, Acharya N. G. Ranga Agricultural University, Lam, Guntur, 522034, Andhra Pradesh, India
²Department of Plant Pathology, Agricultural College, Bapatla, 522101, Acharya N. G. Ranga Agricultural University, Lam, Guntur, 522034, Andhra Pradesh, India
* Corresponding Author : varaprasadakvk@gmail.com

Received : 01-11-2018     Accepted : 27-11-2018     Published : 30-11-2018
Volume : 10     Issue : 11       Pages : 1400 - 1405
Int J Microbiol Res 10.11 (2018):1400-1405

Keywords : Pyricularia grisea, Nutrient medium, Colony diameter, Dry mycelial weight
Conflict of Interest : None declared
Acknowledgements/Funding : Authors are thankful to ICAR-Krishi Vigyan Kendra, Darsi, Prakasam, 523247, Acharya N. G. Ranga Agricultural University, Lam, Guntur, 522034, Andhra Pradesh, India
Author Contribution : All authors equally contributed

Five different solid nutrient media, viz., Potato Dextrose Agar, Czapek Dox Agar, Oat Meal Agar, Rice Polish Agar and Rice Leaf Extract Agar medium evaluated against mycelia growth and sporulation of Pyricularia grisea isolates collected from Prakasam viz., PKM 1 to PKM 6 and Sri Potti Sreeramulu Nellore districts viz., NLR 1 to NLR 6 of Andhra Pradesh. Among the solid media, Oat Meal Agar (79.7mm) followed by Potato Dextrose Agar (79.5mm) supported maximum colony diameter of all isolates while Czapek Dox Agar supported least mycelial growth of P. grisea isolates (65.9mm). The maximum no. of conidia was observed on Oat Meal Agar (3.8X106 conidia/cm2) followed by Rice Polish Agar (3.7X106 conidia/cm2) and no sporulation was observed in Czapek Dox Agar. Among different liquid media viz.,potato dextrose medium, oat meal medium, Czapek Dox medium, rice polish medium and rice leaf extract medium, maximum dry mycelia weight was observed in Potato Dextrose Medium (208.8 mg) followed by Oat Meal Medium (207.0 mg) and least growth was observed on Czapek Dox Medium (84.7 mg).

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