ISOLATION, SCREENING AND CHARACTERIZATION OF MICROORGANISMS PRODUCING PECTINASE ENZYME FROM THE IPOMOEA SPP. AND ITS POTENTIAL APPLICATION

R. SHAIKH¹, J. NAYAK²*, M. MEHTA³, G. SHAH^4
1Department of Biotechnology, Veer Narmad South Gujarat University, Surat, 395 007, Gujarat, India
²Department of Biotechnology, Veer Narmad South Gujarat University, Surat, 395 007, Gujarat, India
³Department of Biotechnology, Veer Narmad South Gujarat University, Surat, 395 007, Gujarat, India
⁴Department of Biotechnology, Veer Narmad South Gujarat University, Surat, 395 007, Gujarat, India
* Corresponding Author : jitendrajayprakash@gmail.com

Received : 24-08-2018     Accepted : 07-09-2018     Published : 30-09-2018
Volume : 10     Issue : 9       Pages : 1355 - 1359
Int J Microbiol Res 10.9 (2018):1355-1359

Keywords : Pectinolytic enzyme, Sweet potato (Ipomoea spp.), SDS-PAGE, Paper chromatography
Conflict of Interest : None declared
Acknowledgements/Funding : The authors are thankful to the Head of the Department, Department of Biotechnology, V.N.S.G. University, Surat-395007; for giving research facilities and encouragement
Author Contribution : All author equally contributed

Pectin is a major constituent of primary cell wall of all land plants and surrounded a range of galacturonic acid rich polysaccharides. Pectinase or pectinolytic enzyme, hydrolyze specific substance, those enzymes have a share of 25% in the global sales of food enzymes. Production of this enzyme is influenced by the various aspects such as nature of solid substrate, level of moisture content, presence or absence of carbon, nitrogen, minerals and vitamin supplements. Maximum (optimum) enzyme production is attained when temperature is around 30°C, pH-8.0, pectin as a carbon source, yeast extract as a nitrogen source and incubation time is 72 h. In addition to this, Sweet potato (Ipomoea spp.) acts as a better agro substrate and for better production of pectinase. Purified pectinase showed a molecular weight of 45-50 kDa observed by means of SDS-PAGE. Paper chromatography results showed that the presence of isolated pectinolytic enzymes. Pectinolytic enzyme is of significant importance in the application of fruit juice industry in the current biotechnological era. The significance of pectinase enzyme is emerging as a rapid fire, since these enzymes act as a tool in the variety of industries like fruit processing industry, vegetables processing industry, paper industry etc., so owing to their vast and variety applications, newer microorganisms are to be screened for the production of pectinase enzyme with desirable properties.

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