EVALUATION OF PROBIOTIC CHARACTERISTICS OF BACTERIA ISOLATED FROM AQUAPONDS OF NELLORE DISTRICT AND THE ASSESSMENT OF ITS EFFICACY IN WHITE LEG SHRIMP INFECTED WITH VIRUS AND WATER QUALITY PARAMETERS IN EXPERIMENTAL CONDITIONS

G. MARY SANDEEPA¹*, K. AMMANI^2
1Department of Biotechnology, Vikrama Simhapuri University, Nellore, 523004, Andhra Pradesh, India
²Department of Micro biology, Acharya Nagarjuna University, Guntur, 522510, Andhra Pradesh, India
* Corresponding Author : deepavsu2013@gmail.com

Received : 24-08-2018     Accepted : 07-09-2018     Published : 30-09-2018
Volume : 10     Issue : 9       Pages : 1341 - 1347
Int J Microbiol Res 10.9 (2018):1341-1347

Keywords : probiotic bacteria, Litopenaeous vannamei, WSSV, lactobacillus
Conflict of Interest : None declared
Acknowledgements/Funding : Author thankful to Acharya Nagarjuna University, Guntur, 522510, Andhra Pradesh, India
Author Contribution : All author equally contributed

In the present study, the probiotic characters of bacteria were determined which were isolated from shrimp cultured ponds. 10 bacterial strains were isolated and probiotic characters were determined. High cholesterol assimilation done by HL5 (27±1.2cfu/ml). V2-V3 region of 16srDNA was amplified for identification of Lactobacillus species. Then experiment was conducted having two phases to investigate the effects of dietary supplementation of probiotic bacterium on shrimp physiology. In first phase shrimp fed with probiotic supplemented feed for 21 days, in second phase challenged with White spot syndrome virus (WSSV and their physiological responses were investigated. In the first phase probiotic treated shrimp had significant (P<0.01) growth, THC, percentage of phagocytosis and phenoloxidase enzyme activity, IgG, IgA,and IgM like substances and antioxidant enzymes . Higher levels were observed in 10% treated shrimp. All three concentrations of Lactobacillus sps probiotic bacterium was effective in improving the resistance of shrimp against WSSV as they had higher THC, higher percentage of phagocytosis, phenoloxidase enzyme and immunoglobin like substances level.

1. Akinbowale OL, Peng H, Barton MD (2006) Journal of Applied Microbiology, 100, 1103–1113.
2. Balcázar JL, Blas ID, Ruiz-Z I, Cunningham D, Vendrell D, Múzquiz JL, (2006). Veternay Microbiology, 114: 173–186.
3. Beauchamp C and Fridovich I (1997) Analytical Biochemistry, 44: 276-287.
4. Bilige M, Liu W, Liping W, Jungowang T, Li H (2009) Journal of Basic microbiology, 59: 493-498.
5. Bishop PA, Smith JF, Kime JC, Mayo JM, Tin TH (1992) International journal of sports medicine, 13: 36-39.
6. Boyd, C. E. (1982 Research and Development Serie, 22pp.
7. Bradford MA (1976) Analytical Biochemistry. 72, 248-254.
8. Burton HS (1956) Journal of Biochemistry, 62, 315–323.
9. Beauchamp C and Fridovich I (1997). Analytical Biochemistry, 4, 276-287
10. Çakır I (2003 Ankara University Thesis of Ph.D.
11. Castex M, Lemaire P, Wabete N, Chim, L (2009) Aqua culture,294, 306-313.
12. Castex M, Lemaire P, Wabete N, Chim L (2010) Fish& Shellfish immunology,28, 622-631.
13. Ceriotti G (1955) Journal of Biochemistry, 214, 59-70.
14. Chance B, Maehly AC, (1995) Methods in enzymology, 2, 764-775.
15. Charteris WP, Kelly PM, Morelli L, Collins JK (1998) Journal of Applied Microbiology, 84, 759-768.
16. Chiu CH, Gua WR, Guu YK, Liu CH, Pan TM, Cheng W (2007) Fish&Shellfish Immunology, 23(2), 364-377.
17. Dellaglio F, Bottazziand V, Vescovo M (1975) International journal of systematic bacteriology, 25, 160-172.
18. FAO (2010) The State of World Fisheries and Aquaculture Rome, 197.
19. Fuller R (1989) Journal of Applied Bacteriology, 66, 365–378.
20. Gilliland SE, Speck ML, Morgan CG (1975) Applied Microbiology, 30, 541-545.
21. Ghosh S, Sinha A, Sahu C(2007) Aquaculture Nutrition, 13: 1–11.
22. Hadi Zokaeifar N, Babaei A, Roos Saad C, Kamarudin MS, Kamaruzaman S, Jose Luis., (2014) Journal of Fish &Shellfish Immunology, 36, 68-74.
23. He S, Zhou Z, Liu Y, Cao Y, Meng K, Shi P, Yao B, Ringo E (2010) Archives of Microbiology 192, 985–994.
24. He S, Zhou Z, Meng K, Zhao H, Yao B, Ringo E, Yoon I (2011) Journal of Animal Science 89, 84–92.
25. He S, Zhou Z, Liu Y, Cao Y, Meng K, Shi P, Yao B, Ringo, E (2012) World Journal of Microbiology & Biotechnology 28,785–791.
26. Hyronimus B, Le Marrec C, Hadi Sassi A, Deschamps A (2000) International Journal of Food Microbiology, 61, 193-197.
27. Jatindra NB, Kouhei O,Yukihiro M,Kozo I (2010)World Academy of Science, Engineering and Technology,71,470-477.
28. Jorge O, Ochoa O, Paniagua-Michel J, Rosalia C (2011) Marine Drugs, 9, 1119-1132.
29. Kondo M (2003) Institute of Applied Aquabiology, National Fisheries University. 1-13.
30. Lavanya B, Sowmiya S, Balaji S, Muthuvelan B (2011) British Journal of Dairy Science. 2(1), 5-10.
31. Liu KF, Chiu CH, Shiu YL, Cheng W, Liu CH (2010) Fish &Shellfish immunology, 28, 837-844.
32. Maragkoudakis PA, Zoumpopoulou G, Miaris C, Kalantzopoulos G, Pot B, Tsakalidou E, (2005 International Dairy Journal.,16, 189-199.
33. Ming-Chaoyu Y, Li ZJ, Lin HZ, Wen GL, Ma S (2008) Aquaculture 16, 471-481.
34. Nagpal R, Kumar A, Kumar M, Behare PV, Jain S, Yadav H (2012) FEMS Microbiology Letters 334, 1–15.
35. Naik PR, Raman G, Narayanan KB, Sakthivel N (2008) BMC Microbiology, 8, 230.
36. Navin Chandran M, Jyapparaj P, Subramanian M, Ramasubburayan R, Santhiyagu P, Immanuel Arunachalam GP (2014) Fish& Shellfish Immunology, 36, 38-45.
37. Padmavathi, P., K. Sunitha and K. Veeraiah. (2012) African Journal of Microbiology Research, 6(49), 7471-7478.
38. Pereira DI, Gibson GR (2002) Applied Environmental Microbiology, 68, 4689–4693.
39. Pope EC., Powell A, Roberts EC, Shields RJ, Wardle R,Rowley AF (2011) PLoS One, 6,20960.
40. Resende J, Silva V, Fontes C, Souza-Filho J, Oliveira T, Coelho C, Cesar D, Dini C (2012) , 27(4), 449-55..
41. Sahul Hameed AS, Anilkumar M, Stephen Raj ML, Jayaraman K (1998) Aquaculture, 160, 31-35.
42. Salminen MK, Tynkkynen S, Rautelin H Saxelin M, Vaara M, Ruutu P, Sarna S, Valtonen V, Jarvinen A (2002) Clinical infectious disease, 35, 1155
43. Schneider C (1957). Methods in Enzymology ,3, 680.
44. Soundarapandian, P. and B. Gunalan. (2008). International Journal of Zoology Reserach,4(1), 21-27.
45. Soundarapandian, P., V. Ramanan and G.K. Dinakaran. (2010) Current Research Journal of Social Sciences, 2(2), 51-57.
46. Serrano PH (2005). FAO (Food and Agriculture Organization of the United Nations) Fisheries Technical Paper. 469.
47. Sung HH, Kou GH, SongYL (1994) Fish Pathology, 29, 11-17.
48. Svetoslav D, Todorov D, Johan W, Mollendroff V, Muller EMN, Wittuhuhn RC, Dicks, LMT(2009). Biotechnology, 47,178-191.
49. Strompfova V, Laukova A (2007) Anaerobe, 13, 228–237.
50. Wang WQ, Li AJ, Lan CX, Guo J, (1998). Journal of Fish China, 22(2), 170-174.
51. Wang YB, Li JR, Lin J (2008) Aquaculture, 281, 1–4.
52. Wang XW, Wang JX( 2013). Fish & shell fish immunology, 34(4), 981-989.
53. Watson AK, Kaspar H, LateganMJ, Gibson L (2008) Aquaculture , 274, 1–14.
54. Weeks-Perkins BA, Chansue N,Wong-Verelle D, Stolen JS, Fletcher TC, Smith SA, Zelikoff, JT, Kaattari R, Anderson A, So¨derha¨ll K, Weeks-Perkins BA (1995) Technics in Fish Immunology, 4,223–231.
55. Yeh M.S, Lai CY, Liu CH, Kuo CM, Cheng W (2009) Fish& Shellfish Immunology, 26, 49–55.
56. Zhou XX, Wang YB, Li, WF (2009) Aquaculture , 287, 349-353