COMPARATIVE STUDIES ON POCK MORPHOLOGY AND ANTIGENIC PROPERTIES OF AVIPOXVIRUSES

V.R. GILHARE¹*, S.D. HIRPURKAR², A. JAIN³, N. RAWAT^4
1Department of Veterinary Anatomy, College of Veterinary Science and Animal Husbandry, Durg, 491001, Chhattisgarh Kamdhenu Vishwavidyalaya, Raipur, 492006, India
²Department of Veterinary Anatomy, College of Veterinary Science and Animal Husbandry, Durg, 491001, Chhattisgarh Kamdhenu Vishwavidyalaya, Raipur, 492006, India
³Department of Animal Genetics and Breeding, College of Veterinary Science and Animal Husbandry, Durg, 491001, Chhattisgarh Kamdhenu Vishwavidyalaya, Raipur, 492006, Chhattisgarh, India
⁴Department of Veterinary Anatomy, College of Veterinary Science and Animal Husbandry, Durg, 491001, Chhattisgarh Kamdhenu Vishwavidyalaya, Raipur, 492006, India
* Corresponding Author : docvarsha20@gmail.com

Received : 09-07-2018     Accepted : 23-07-2018     Published : 30-07-2018
Volume : 10     Issue : 7       Pages : 1314 - 1320
Int J Microbiol Res 10.7 (2018):1314-1320

Keywords : Virus, Passage, Chorioallantoic membrane, Pock, Polypeptide band
Conflict of Interest : None declared
Acknowledgements/Funding : Authors are thankful to College of Veterinary Science and Animal Husbandry, Durg, 491001, Chhattisgarh Kamdhenu Vishwavidyalaya, Raipur, 492006, Chhattisgarh, India and Indian Council of Agricultural Research, New Delhi, India for Dev. grant 2016-17.
Author Contribution : S.D. Hirpurkar designed the experiment. Varsha Rani Gilhare carried out the experiment and drafted the final manuscript. Asit Jain helped in analysis and Nidhi Rawat helped in the sample collection.

During the present study five isolates i.e., three of fowlpox virus (FPV), one of turkey pox virus (TPV) and one of pigeonpox (PPV) was collected from naturally pox infected chickens, turkey and pigeon in different outbreaks attended in various districts of Chhattisgarh state. Virus was propagated in chicken embryo upto 20 serial passage to ensure the successful adaptation of each virus to chorioallantoic membrane (CAM). Appearance of pock lesions and concentration of virus was inconsistent during initial passage (from 1 to 3 passage), in later passages (10 to 20 passage) the low degree of variation in macroscopic appearance of pock lesions was observed, also the pock morphology was consistent irrespective of Avipoxvirus (APV) strain. Partially purified field isolates of one each of FPV, TPV, PPV and vaccine strain of FPV (Vac) were subjected to Western blotting and the protein profile of five field isolates was compared with that of commercial FPV vaccine strain. All field strain of APVs, except PPV showed common polypeptide bands with size of 22 kDa, 35 kDa, 39 kDa, 48 kDa, 62 kDa, 65 kDa, 70 kDa and 91 kDa. While protein profile PPV showed extra bands with size of 27 kDa. These bands were also found in FPV (Vac) but 22 kDa was missing.

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