DETECTION AND GENOTYPIC CHARACTERIZATION OF ROTAVIRUS FROM DIARRHOEIC PIGLETS OF ARUNACHAL PRADESH, A NORTH EASTERN STATE OF INDIA

G.B. GARAM¹, D.P. BORA²*, M. BORA³, S.P. AHMED⁴, S.K. DAS⁵, Y.P.S. MALIK^6
1Department of Microbiology, College of Veterinary Science, Assam Agricultural University, Guwahati, 781022, Assam, India
²Department of Microbiology, College of Veterinary Science, Assam Agricultural University, Guwahati, 781022, Assam, India
³Department of Microbiology, College of Veterinary Science, Assam Agricultural University, Guwahati, 781022, Assam, India
⁴Department of Veterinary Public Health, College of Veterinary Science, Assam Agricultural University, Guwahati, 781022, Assam, India
⁵Department of Microbiology, College of Veterinary Science, Assam Agricultural University, Guwahati, 781022, Assam, India
⁶Division of Biological Standardization, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, 243122, Uttar Pradesh, India
* Corresponding Author : drdpbora@gmail.com

Received : 20-05-2018     Accepted : 27-05-2018     Published : 30-05-2018
Volume : 10     Issue : 5       Pages : 1228 - 1231
Int J Microbiol Res 10.5 (2018):1228-1231
DOI : http://dx.doi.org/10.9735/0975-5276.10.5.1228-1231

Keywords : Piglets, Group A rotavirus, RNA-PAGE, RT-PCR, Nested-multiplex PCR, G-genotyping, P-genotyping
Conflict of Interest : None declared
Acknowledgements/Funding : The authors gratefully acknowledge the Department of Biotechnology, Govt. of India for providing necessary facilities under the project entitled “Development of Improved Diagnostics with Monitoring and Characterization of Viral and Bacterial Pathogens Associated with Piglet Diarrhoea in North Eastern Region of India” to carry out the research work [Grant No. BT/168/NE/TBP/2011]. Also, GBG acknowledges the Veterinary and Animal Husbandry Department, Government of Arunachal Pradesh for providing study leave to carry out the research work
Author Contribution : This study was a part of GBG’s research work during his M.V.Sc. program. GBG carried out the experiment. DPB, SKD and YPSM designed the experiment and provided necessary guidelines. MB and SPA assisted in laboratory works. DPB drafted the final manuscri

Rotavirus (RV) has been considered as one of the most important cause of severe gastroenteritis among human infants and the neonates of most farm animal species worldwide. In piglets, RV diarrhoea has been identified as a major cause of mortality and morbidity. The present study was undertaken to detect rotavirus from diarrhoeic faecal samples of pig population of Arunachal Pradesh and to investigate genotypic characterization of the same. A total of 342 faecal samples were collected from diarrhoeic piglets of different places of Arunachal Pradesh. The samples were screened for presence of RV by RNA-PAGE and RT-PCR with amplification of VP4 and VP7 genes of RV. Further, genotyping of the RV circulating among the pig population of Arunachal Pradesh was carried out to find out the G-and P-types of RV detected from diarrhoeic piglets. Of 342 diarrhoeic faecal samples, 53 (15.49%) and 127 (37.13%) samples were found positive for group A RV (GARV) in RNA-PAGE and RT-PCR respectively. On genotyping, it was observed that, major G-P combination circulating among the pig population Arunachal Pradesh was G10P[7] (33.3%) followed by G10P[8](8.33%) and G9P[8] (8.33 %). Most of the samples (50.00 %)were found to have mixed type of G-P combinations. To the authors’ knowledge, this is the first report of genotypic characterization of RV from Arunachal Pradesh, India.

1. Kapikian A.Z., Flores J., Hoshino Y., Glass R.I., Midthunk K., Gorziglia M. and Chanock R.M. (1986) J. Infect. Dis., 153, 815-822.
2. Dhama K., Chauhan, R.S., Mahendran, M. and Malik, S.V.S. (2009) Vet. Res. Commun., 33, 1-23.
3. Martella V., Banyai K., Matthijnssens J., Buonavoglia C. and Ciarlet M. (2010) Veterinary Microbiol., 140, 246-255.
4. Broor S., Ghosh D. and Mathur P. (2003) Indian J. Med. Res., 118, 59-67.
5. Li Z., Baker M.L., Jiang W., Estes M.K. and Prasad B.V. (2009) J. Virol., 83(4), 1754-1766.
6. Ciarlet M. and Estes M.K. (1999) J. Gen. Virol. 80, 943-948.
7. Estes M.K. (2001) In, Field Virology. Knipe, D.M. and Howley, P.M. (eds.), 4th edn., Lippincott Williams and Wilkins, Philadelphia, PA. pp. 1747-1785.
8. Estes M.K. and Cohen J. (1989) Microbial. Rev., 53, 410-449.
9. Matthijnssens J., Otto P.H., Ciarlet M., Desselberger U., Van Ranset M. and Johne R. (2012) Arch. Virol., 157, 1177-1182.
10. Tayo T., Lonhjam N. and Perme B. (2011) Vet. World, 4 (7),332-336.
11. Malik Y.P.S., Kumar N., Sharma K., Bora D.P. and Dutta T.K. (2014) Ind J. Anim. Sci., 84(10), 1035–1042.
12. Bora D.P., Bora M., Borah B., Bezborah B., Sarma D. K., Malik Y P S. and Dutta T. K. (2014) J Immunol.Immunopathol.,16 (1&2), 53-57.
13. Mondal A., Sharma K., Malik Y.S., Joardar S.N. (2013) Adv. Anim. Vet. Sci., 1 (1S), 18 – 19.
14. Bora D.P., Barman N.N. & Bhattacharyya D.K. (2007) Ind. J Virol., 18, 40-43.
15. Barman N.N., Barman B., Sharma D.K. and Pensaert M.B. (2003) Indian J. Anim. Sci., 73(6), 576-578.
16. Herring A.J., Inglis N.F., Ojelt C.K., Snodgrass D.R. and James D. (1982) J. Clin. Microbiol., 16, 473-477.
17. Gouvea V., Glass R.I., Woods K., Taniguchi K., Clarke H.F., Forrester B. and Fang Z.Y. (1990) J. Clin. Microbiol., 28, 276-282.Gouvea V., Santos N. and Timenetsky M. do C. (1994) J. Clin. Microbiol., 32, 1338-1340.
18. Gentsch J.R., Glass R.I., Woods P., Gouvea V., Gorziglia M., Flores J., Das B.K., and Bhan M.K. (1992) J Clin Microbiol., 30 ,1365-1373.
19. Matthijnssens J., Ciarlet M., Rahman M., Attoui H., Banyai K., Estes M. K., Gentsch J. R., Iturriza-Gomara M., Kirkwood C. D., Martella V., Mertens P. P., Nakagomi O.,Patton J. T., Ruggeri F. M., Saif L. J., Santos N., Steyer A., Taniguchi K., Desselberger U. and van Ranst M. (2008) Archives of virology, 153, 1621-9.
20. Bora D.P., Barman N.N., Bhattacharyya D.K., Sarma S. and Dutta L.J. (2010) Ind. Vet. J., 87,641-643.
21. Gatti M.S.V., Ferraz M.M.G., Racz M.L. and de Castro A.F.P. (1993) Vet. Micobiol., 37, 187-190.
22. Sarkar J.K. and Soman J.P. (1994) Indian J. Anim. Sci. 64, 907-913.
23. Sharma R., Bora D. P., Chakraborty P., Das S., Barman N.N. (2013) Indian Journal of Virology, 24 ,250-255.
24. Falcone E., Tarantino M., Trani L.D., Cordioli P., Lavazza A. and Tollis M. (1999) J. Clin. Microbiol., 37, 3879-3882.
25. Ciarlet M., Liprandi F. (1994) J. Clin. Microbiol. 32, 269–272.
26. Ramos A.P., Stefanelli C.C., Linhares R.E., De Brito B.G. and Nozaw C.M. (1998) Braz. J.Vet. Res., 35(2), 84-87.
27. Steele A.D., Geyer A. and Gerdes G.H. (2004) Infectious diseases of Livestock. Pp. 1256–64. (Eds) Coetzer JAW and Tustin RC, Oxford University Press, Southern Africa.
28. Papp H., Laszlo B., Jakab F.,Ganesh B., De Grazia S., Matthijnssens J., Ciarlet M.,Martella V. and Banyai K. ( 2013) Vet. Microbiol., 165,190-199
29. Varghese V., Das S., Singh N.B., Kojima K., Bhattacharya S.K., Krishnan T., Kobayashi N. and Naik T.N. (2006) Arch.Virol., 149,155–62.
30. Kusumakar A.L., Savita, Malik Y.P.S., Minakshi and Prasad G. (2008) Ind. J. Anim. Sci., 78 (5), 475–77.
31. Chitambar S.D., Arora R., Kolpe A.B., Yadav M.M., Raut C.G. (2011) Vet. Microbiol., 148, 384–388.
32. Mukherjee A., Dutta D., Ghosh S., Bagchi P., Chattopadhyay S., Nagashima S., Kobayashi N., Dutta P., Krishnan T., Naik T.N., Chawla-Sarkar M. (2009) Arch. Virol., 154, 733–746.
33. Mukherjee A., Ghosh S., Bagchi P., Dutta D., Chattopadhyay S., Kobayashi N., Chawla-Sarkar M. (2011) Clin. Microbiol. Infect., 17, 1343–1346.
34. Hardy M.E., Gorziglia M. and Woode G.N. (1992) Virology, 191,291-300.