MOLECULAR AND ANTIBODY BASED DIAGNOSIS OF DUCK PLAGUE VIRUS FROM FIELD OUTBREAKS IN ASSAM, INDIA

M.K. DOLEY¹*, S.K. DAS², N.N. BARMAN³, N. BARUAH^4
1ICAR-Krishi Vigyan Kendra, Karbi Anglong, Assam Agricultural University, Diphu, 782462, India
²Department of Veterinary Microbiology, Assam Agricultural University, Guwahati, 781022, India
³Department of Veterinary Microbiology, Assam Agricultural University, Guwahati, 781022, India
⁴Regional Agricultural Research Station, Assam Agricultural Research Station, Diphu, 782462, India
* Corresponding Author : monujdoley201039@gmail.com

Received : 15-05-2018     Accepted : 27-05-2018     Published : 30-05-2018
Volume : 10     Issue : 5       Pages : 1220 - 1223
Int J Microbiol Res 10.5 (2018):1220-1223
DOI : http://dx.doi.org/10.9735/0975-5276.10.5.1220-1223

Keywords : Duck plague virus, DPV, S-ELISA, AGPT, CIE, PCR
Academic Editor : Dr Bern Ozbek Celik
Conflict of Interest : None declared
Acknowledgements/Funding : Author thankful to Department of Veterinary Microbiology, College of Veterinary Science, Assam Agricultural University, Khanapara, 781022, India
Author Contribution : All author equally contributed

The study was undertaken to detect duck plague virus by PCR and antibody-based techniques. A total of 79 samples were collected from clinically affected (40) and dead ducks (39). PCR detects presence of the virus in 85.00% and 87.17% from clinically affected and dead birds respectively. Among the antibody-based techniques, S-ELISA was found more sensitive in comparison to other tests showing positive results 32.05% and 71.79% from clinically affected and dead birds respectively. AGPT and CIE could detect the virus only in post mortem samples. Both AGPT and CIE were found to be equally sensitive giving positive result of 12.82% from dead ducks. Liver and spleen samples from dead birds were found best for pathogen detection. In cases of clinically affected ducks, whole blood showed to be better results than the cloacal swabs.

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