EVALUATION OF TYROSINASE PRODUCING INDIGENOUS BACTERIAL STRAINS WITH REFERENCE TO THEIR EFFICACY IN L-DOPA PRODUCTION

ANJANA SHARMA¹*, PRASHANSA VYAS², MEENAL BUDHOLIA REHMAN^3
1Bacteriology Laboratory, Department of P. G. Studies and Research in Biological Science, Rani Durgavati University, Jabalpur, 482001, Madhya Pradesh, India
²Bacteriology Laboratory, Department of P. G. Studies and Research in Biological Science, Rani Durgavati University, Jabalpur, 482001, Madhya Pradesh, India
³Department of Botany, Mata Gujri Women’s College, Jabalpur, Madhya Pradesh, 482001, India
* Corresponding Author : anjoo1999@gmail.com

Received : 14-05-2018     Accepted : 24-05-2018     Published : 30-05-2018
Volume : 10     Issue : 5       Pages : 1202 - 1204
Int J Microbiol Res 10.5 (2018):1202-1204
DOI : http://dx.doi.org/10.9735/0975-5276.10.5.1202-1204

Keywords : Tyrosinase, L-Dopa, Drug, L- Tyrosine, Soil, identification
Conflict of Interest : None declared
Acknowledgements/Funding : Authors are thankful to Department of P. G. Studies and Research in Biological Science, Rani Durgavati University, Jabalpur, 482001, Madhya Pradesh, India
Author Contribution : Author Contributions: All

The present study is focussed on isolation and screening of tyrosinase enzyme produced by bacterial isolates and its application in the production of L-Dopa. L- Dopa is an amino acid which is used as a drug for the treatment of Parkinson’s disease. 112 bacterial isolates have been screened which were collected from soil of different gardens of Jabalpur, Madhya Pradesh. Tyrosinase activity of all bacterial isolates was determined qualitatively and quantitatively. The primary screening of all the isolates for Tyrosinase production was carried on Tyrosine agar medium. Among them 21 isolates showed brown pigmented colonies indicating Tyrosinase activity. Secondary screening was done on the basis of extracellular enzyme activity. Based on the result of secondary screening, out of 21 isolates 5 were reported as potential Tyrosinase producers which were further checked for L-Dopa production. On the basis of morphological, cultural and biochemical characteristics the potent L-Dopa producing bacterial isolate was identified as Pseudomanas sp which could be a promising source for the production of L-Dopa.

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