ISOLATION AND SCREENING FOR MULTI-TRAIT PLANT GROWTH PROMOTING ACTINOBACTERIA FROM ORGANIC SUGARCANE RHIZOSPHERE

ARIANA ALVES RODRIGUES¹*, MARCUS VINICIUS FORZANI ARAUJO², RENAN DE SOUZA SOARES³, BRUNO FRANCESCO RODRIGUES DE OLIVEIRA⁴, SERGIO TADEU SIBOV⁵, JOSE DANIEL GONCALVES VIEIRA^6
1Laboratory of Environmental Microbiology and Biotechnology (LAMAB), Institute of Tropical Pathology and Public Health, Federal University of Goias, Goiania, Goias, 74605050, Brazil
²Laboratory of Environmental Microbiology and Biotechnology (LAMAB), Institute of Tropical Pathology and Public Health, Federal University of Goias, Goiania, Goias, 74605050, Brazil
³Laboratory of Environmental Microbiology and Biotechnology (LAMAB), Institute of Tropical Pathology and Public Health, Federal University of Goias, Goiania, Goias, 74605050, Brazil
⁴Laboratory of Molecular and Marine Bacteriology, Institute of Microbiology Paulo de Goes, Federal University of Rio de Janeiro, Rio de Janeiro, 74690900, Brazil
⁵Laboratory of Tissue Culture, School of Agronomy, Federal University of Goias, Goiania, Goias, 74605050, Brazil
⁶Laboratory of Environmental Microbiology and Biotechnology (LAMAB), Institute of Tropical Pathology and Public Health, Federal University of Goias, Goiania, Goias, 74605050, Brazil
* Corresponding Author : ariana.biomed@gmail.com

Received : 27-03-2018     Accepted : 21-05-2018     Published : 30-05-2018
Volume : 10     Issue : 5       Pages : 1193 - 1198
Int J Microbiol Res 10.5 (2018):1193-1198
DOI : http://dx.doi.org/10.9735/0975-5276.10.5.1193-1198

Keywords : Rhizosphere, Organic Sugarcane, Phosphate Solubilization, Nitrogen Free Medium, Streptomyces
Academic Editor : Raylane Pereira Gomes
Conflict of Interest : None declared
Acknowledgements/Funding : The authors would like to thank Jalles Machado and Goiasa-Goiatuba Álcool Ltda for allowing and assisting the collection of rhizospheric soil, Centro de Tecnologia Canavieira (CTC) for providing the Fusarium strain used in this study. Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Capes) for providing scholarship to the first author and the Graduate Program in Environmental Sciences (CIAMB) for financial assistance.
Author Contribution : All author equally contributed

Rhizospheric actinobacteria associated to organic cultivation are essential in nutrient cycling and plant growth promotion. The aim of this study was to isolate and select in vitro actinobacteria displaying multiple plant growth promoting traits associated with the rhizosphere of sugarcane undergoing organic management. The isolates were evaluated regarding their ability to produce plant growth promoting traits. Of the 21 isolates, 57% produced at least one of the evaluated traits. Isolates ABC92 and ABC32 produced 60.28 and 55.36 μg mL-1 of fitohormone in 21 days, whereas isolates ANC48 and ANU34 were the best solubilizers, solubilizing 8.93 and 8.92 μg mL-1 phosphate. A total of 29% of the microorganisms were able to grow in nitrogen-free media and 24% were ammonia producers. Isolates ABC31, ANC48 and ANU49 were able to inhibit Fusarium moniliforme growth. All actinobacteria identified in this study belonged to the Streptomyces genus and presented potential as plant growth promoting agents.

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