REPORT OF Turnip mosaic virus OCCURRENCE IN BROAD LEAVED MUSTARD (Brassica juncea var. Rugosa) FROM MANIPUR, INDIA

NABAKISHOR NONGMAITHEM¹*, THIYAM REBIKA^2
1Directorate of Research, Central Agricultural University, Imphal, 795004, Manipur, India
²Colleges of Agriculture, Central Agricultural University, Imphal, 795004, Manipur, India
* Corresponding Author : nabaaaidu@yahoo.com

Received : 12-05-2018     Accepted : 23-05-2018     Published : 30-05-2018
Volume : 10     Issue : 5       Pages : 1191 - 1192
Int J Microbiol Res 10.5 (2018):1191-1192
DOI : http://dx.doi.org/10.9735/0975-5276.10.5.1191-1192

Keywords : Broad leaved mustard, Turnip mosaic virus, Symtomatology, Disease incidence, RT-PCR
Conflict of Interest : None declared
Acknowledgements/Funding : Author thankful to Central Agricultural University, Imphal, 795004, Manipur, India
Author Contribution : All author equally contributed

The occurrence of Turnip mosaic virus (TuMV) in broad leaved mustard (Brassica juncea var. Rugosa) grown in Manipur, India was confirmed by symptomatology and reverse transcription- polymerase chain reaction (RT-PCR). Symptoms showing mosaic, mottling, interveinal chlorosis, irregular chlorotic patches and puckering were observed on broad leaved mustard with maximum mean disease incidence of 42.12%. RT-PCR assay was carried out using potyvirus-specific degenerate primers NIb2F and NIb3R, targeting the core region of the NIb. The RT-PCR assays using NIb specific primers produced amplicon of 350bp, only in the symptomatic leaf samples. This is the first molecular evidence of TuMV infection in broad leaved mustard from Manipur, India.

1. Ahlawat Y.S. and Chenulu V.V. (1984) Tropical Agriculture, 61, 88-92.
2. Devi S.P., Bhat A.I., Devi L.R., Das M.L. (2004) Indian Phytopathology, 57(4), 488-93.
3. Haq Q.M.R., Srivastava K.M., Raizada R.K., Singh B.P., Jain R.K., Mishra A, Shukla D.D. (2008) Journal of Phytopathology, 140,55-64.
4. Kulkarni G.S. (1924) Poona Agriculture College Magazine, 16, 6–12.
5. Babu B., Dankers H., George S., Wright D., Marois J. and Paret M. (2013) Plant disease, 97(12), 1664
6. Raghuveer Singh, Amrita Banerjee, Susheel S.K., Bhagawati R., Sikimoni B., Ngachan S.V. (2015) Virus Diseases, 26(3), 211-213.