FIRST DETECTION OF AMINOGLYCOSIDES RESISTANCE GENES AAC(6)-IB, ANT(2)-I AND AAD IN ENTEROBACTERIACEAE PRODUCING EXTENDED-SPECTRUM BETA-LACTAMASES IN ABIDJAN (COTE DIVOIRE)

VICTOIRE GADOU¹*, NATHALIE KOUADIO GUESSENND², ABALE A. TOTY³, SEYDINA M. DIENE⁴, JEAN MARC ROLAIN⁵, JOSEPH ALLICO DJAMAN⁶, MIREILLE DOSSO^7
1Laboratory of Biochemical Pharmacodynamics, Department of Biosciences, Felix Houphouet Boigny University, Abidjan, 22 BP 582 Abidjan 22 Cote dIvoire
²Pasteur Institute of Cote d Ivoire, 08 BP 1563 Abidjan 08
³Pasteur Institute of Cote d Ivoire, 08 BP 1563 Abidjan 08
⁴Research Unit on Emerging Tropical Infectious Diseases of Aix-Marseille University, IHU Mediterranee Infection, Faculty of Medicine and Pharmacy, Aix-Marseille-University, 27 Bd Jean Moulin, 13385 Marseille Cedex 5, France
⁵Research Unit on Emerging Tropical Infectious Diseases of Aix-Marseille University, IHU Mediterranee Infection, Faculty of Medicine and Pharmacy, Aix-Marseille-University, 27 Bd Jean Moulin, 13385 Marseille Cedex 5, France
⁶Laboratory of Biochemical Pharmacodynamics, Department of Biosciences, Felix Houphouet Boigny University, Abidjan, 22 BP 582 Abidjan 22 Cote dIvoire
⁷Pasteur Institute of Cote d Ivoire, 08 BP 1563 Abidjan 08
* Corresponding Author : victoiregadou@yahoo.fr

Received : 19-04-2018     Accepted : 08-05-2018     Published : 30-05-2018
Volume : 10     Issue : 5       Pages : 1171 - 1174
Int J Microbiol Res 10.5 (2018):1171-1174
DOI : http://dx.doi.org/10.9735/0975-5276.10.5.1171-1174

Keywords : Enterobacteriaceae, ESBL, aminoglycosides, resistance, Abidjan
Conflict of Interest : None declared
Acknowledgements/Funding : We are grateful to the Ministry of Higher Education and Scientific Research of Côte d'Ivoire for having given a scholarship in France (No. 912 / MESRS / DB / SD-BHCI / SD / CBK). We also thank Mr. Jean Marc Rolain in Research unit on emerging tropical infectious diseases for his grateful assistance and technical support.
Author Contribution : All author equally contributed

The aim of this study was to highlight the presence of aminoglycoside resistance genes in enterobacteriaceae producing extended-spectrum beta-lactamases isolated in Abidjan. The study involved 153 enterobacteriaceae of human origin and whose identification has been confirmed by Maldi Tof-type Mass Spectrometry. The antibiotic susceptibility testing was performed by diffusion on Mueller-Hinton E agar. The beta-lactams resistance genes were characterized by real-time PCR, conventional PCR and sequenced. While the aminoglycoside resistance genes were detected through conventional PCR directly. Of these strains 90 (58.8%) were producing broad-spectrum beta-lactamase. A high resistance rate to aminoglycosides (90%), cefotaxime (95.6%), ceftriaxone (96.7%), and cefoxitin (72.2%) was observed in enterobacteriaceae producing extended-spectrum beta-lactamases. The aminoglycoside resistance genes found were aac (6) -Ib, ant (2 ") –I and aad at the rate of 58.9%, 8.9% and 7.8% respectively. Resistance genes to β-lactams detected were blaCTX-M (96.7%), blaTEM (67.8 %) and blaSHV (27.8%). This study is the first to describe the aminoglycoside resistance genes in clinical strains of enterobacteriaceae in Abidjan.

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