GENETIC CONFIRMATION OF MUNGBEAN GENOTYPES (Vigna radiata L. WILCZEK) USING MOLECULAR MARKERS

ANAMIKA NATH¹*, S.R. MALOO², DEVENDRA JAIN³, SRIKANTA NATH⁴, ASHIM CHAKMA⁵, RAJANI VERMA^6
1Department of Plant Breeding and Genetics, Rajasthan College of Agriculture, Maharana Pratap University of Agriculture and Technology, Udaipur, 313001, Rajasthan
²Department of Plant Breeding and Genetics, Rajasthan College of Agriculture, Maharana Pratap University of Agriculture and Technology, Udaipur, 313001, Rajasthan
³Department of Molecular Biology and Biotechnology, Rajasthan College of Agriculture, Maharana Pratap University of Agriculture and Technology, Udaipur, 313001, Rajasthan
⁴Department of Agriculture, O/o The Supdt. Of Agriculture, Govt. of Tripura, Gandacherra, 799284, Tripura
⁵Department of Agriculture, O/o The Supdt. Of Agriculture, Govt. of Tripura, Gandacherra, 799284, Tripura
⁶Department of Plant Breeding and Genetics, Rajasthan College of Agriculture, Maharana Pratap University of Agriculture and Technology, Udaipur, 313001, Rajasthan
* Corresponding Author : anamikanath22@gmail.com

Received : 31-03-2018     Accepted : 05-04-2018     Published : 15-04-2018
Volume : 10     Issue : 3       Pages : 365 - 369
Genetics 10.3 (2018):365-369
DOI : http://dx.doi.org/10.9735/0975-2862.10.3.365-369

Keywords : Mungbean, RAPD Marker, ISSR Markers, and yield components
Conflict of Interest : None declared
Acknowledgements/Funding : The Author thankful to department of Plant Breeding and Genetics, Rajasthan College of Agriculture, Maharana Pratap University of Agriculture and Technology, Udaipur, 313001, Rajasthan. The authors would like to acknowledge Dr S. R. Maloo, Former Dean, Rajasthan College of Agriculture, Maharana Pratap University of Agriculture and Technology, Udaipur, 313001, Rajasthan for help in conducting the research work successfully.
Author Contribution : All author equally contributed

The present study was undertaken to identify the genetic variation present among different mungbean genotypes. Out of 25 RAPD primers only 17 were amplified. A total of 104 amplified bands, 91 polymorphic, 13 monomorphic bands and 88 % polymorphism. A total of 112 amplified bands were obtained from 18 ISSR primers, out of which 88 polymorphic. The average number of bands per primer was 6.22 and average numbers of polymorphic bands per primer 4.89. The RAPD and ISSR data were evaluated to obtain a combined similarity matrix and cluster tree analysis. The similarity coefficient values lay between 0.46-0.68 and cluster tree analysis showed that the eight genotypes could be divided into 4 clusters. The genotype BM-4 was grouped in separate VI cluster and PDM-139 was grouped on cluster IIA. In the light of RAPD and ISSR study the parents of the cross BM-4 x PDM-139 noticed for their genetic diversity. Here validation of these data compared with phenotypic character in the field and revealed that cross BM-4 x PDM-139 turned out to be the most promising on the basis of its high per se performance and also for their high genetic diversity.

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