MOLECULAR ANALYSIS OF BLAST RESISTANCE GENES IN RICE USING GENE LINKED AND GENE BASED MARKERS

H.B. MANOJ KUMAR¹*, C.A. DEEPAK², M.P. RAJANNA³, K.M. HARINI KUMAR⁴, MUNAWERY AYEESHA^5
1Department of Plant Biotechnology, University of Agricultural Sciences, GKVK, Bengaluru, 560065, Karnataka
²ICAR-ZARS, V.C. Farm, Mandya, 571405, University of Agricultural Sciences, GKVK, Bengaluru, 560065, Karnataka
³ICAR-ZARS, V.C. Farm, Mandya, 571405, University of Agricultural Sciences, GKVK, Bengaluru, 560065, Karnataka
⁴Department of Plant Biotechnology, University of Agricultural Sciences, GKVK, Bengaluru, 560065, Karnataka
⁵ICAR-ZARS, V.C. Farm, Mandya, 571405, University of Agricultural Sciences, GKVK, Bengaluru, 560065, Karnataka
* Corresponding Author : manojmandya.17@gmail.com

Received : 14-02-2017     Accepted : 27-02-2017     Published : 28-02-2017
Volume : 9     Issue : 2       Pages : 248 - 251
Genetics 9.2 (2017):248-251

Keywords : Rice, marker validation and blast resistant genes
Conflict of Interest : None declared
Acknowledgements/Funding : Authors are gratefully acknowledging the financial assistance provided by RKVY, GOI to carry out this study under the project entitled “Introgression of Multiple Resistance Genes and Developing Blast Resistant Rice Variety Suitable for Southern Karnataka” and DBT, GOI.
Author Contribution : Experiment was designed by Deepak, C.A. Rajanna, M.P. and Harini Kumar, K.M Complete lab work has been performed by Manoj Kumar, H.B. and Ayeesha Munawery. All authors read and approved the final manuscript

Rice blast caused by Magnaporthe oryzae, spread in more than 85 countries and has caused great yield loss. Development and growing of rice varieties would be the most effective way to control blast disease. Molecular analysis and major rice blast resistance genes for genetic diversities were determined and molecular characterization (or) screening of major rice blast resistance genes was determined with molecular markers, which showed close set linkage to 10 major rice blast resistance genes (Pi-54, Pi-1, Pi-2, Pi-9, Pik, Pikm, Pitp, Pi-38, Pizt and Pi 7t), in a collection of 15 accession (including some varieties). Out of the 15, the Pikm, Pizt and Pi7t appeared to be widely present in all the varieties with respect their resistant allele size and gave positive express. For the gene, Pi-54, Pi-1, Pi-2, Pi-9, Pik, Pitp and Pi-38 gene frequencies were 33.33 %, 40.01%, 86.67%,6.67%, 60.07%,33.33% and 33.33% respectively. Among the 15 accessions, 5 were positive for Pi-54, Pitp and Pi-38 genes and six accessions were positive for Pi-1 gene, thirteen for Pi-2, nine for Pikm and one for Pi-9 gene. Four accessions viz., BR 2655, JAYA, RAKSHA and BASUMATHI-370 were positive for two major and broad spectrum genes i.e Pi-54 and Pi-2. Out of 15, only three accessions namely BR-2655, JAYA and BASUMATHI-370 were detected with maximum number (> 7) of genes. Less number of genes (<4) harboured in KMP-200, KMP-201 and IR-64. These results are useful in identification and incorporation of functional resistance genes from these evaluated varieties into elite cultivars through marker-assisted selection for improved blast resistance in India and worldwide.