METAGENOMIC ANALYSIS OF BACTERIAL COMMUNITY IN SOIL USING 16S rRNA GENE THROUGH HIGH THROUGHPUT SEQUENCING

S. GUPTA¹*, S.K. CHIKARA², P. RISHISHWAR^3
1Department of Biotechnology, Shri Venkateshwara University, Rajabpur Gajraula, Uttar Pradesh, 244236, India
²Department of Biotechnology, Shri Venkateshwara University, Rajabpur Gajraula, Uttar Pradesh, 244236, India
³Department of Biotechnology, Shri Venkateshwara University, Rajabpur Gajraula, Uttar Pradesh, 244236, India
* Corresponding Author : shobit.jhs@gmail.com

Received : 11-07-2016     Accepted : 21-07-2016     Published : 28-07-2016
Volume : 8     Issue : 3       Pages : 190 - 193
Genetics 8.3 (2016):190-193

Keywords : Soil, 16S rRNA gene, High throughput sequencing, rRNA
Academic Editor : Dr K M Singh, Dr Rudra Prasanna Panda
Conflict of Interest : None declared
Acknowledgements/Funding : The authors are thankful to Director and Management of Xcelris labs, Ahmedabad, Gujarat, India for providing funding to this work.
Author Contribution : None declared

Bacterial 16S ribosomal DNA (rDNA) amplicons have been widely used in the classification of uncultured bacteria inhabiting environmental niches. Primers targeting conservative regions of the rDNAs are used to generate amplicons for metagenomics study. The present study applied metagenomics to characterize the diversity and relative occurrence of Bacterial organisms in the soils of Chandan plant (MS1) and Bamboo plant (MS2) using high throughput sequencing. The metagenome samples were subjected to sequencing by Ion torrent PGM which resulted in 1,418,770 (MS1) and 1,695,228 (MS2) reads respectively. The taxonomic profile obtained by comparison with M5NR database showed predominance of Actinobacteria, Proteobacteria and Firmicutes in both the samples, abundance of Actinobacteria in MS-1(46.84%) and in MS-2(47.06%), followed by Proteobacteria in MS-1(26.80%) and in MS-2(26.91%) , Firmicutes in MS-1(23.11%) and MS-2(22.79%). At genus level, metagenomics revealed 16 genera in soil 1 (MS1) and 18 genera in soil 2 (MS2), Mycobacterium is predominant in both the samples, in MS-1(33.18%) and in MS-2 (32.97%). The present study provides a preliminary snapshot of the diversity and relative abundance of the bacteria within the soil samples and expands our knowledge of these dynamic Bacterial communities present in the soil ecosystem and these consortiums may be helpful in soil fertility and enhance plant productivity.