PREVALENCE, BIOCHEMICAL CHARACTERIZATION AND MOLECULAR DETECTION OF STAPHYLOCOCCUS AUREUS IN DIFFERENT CLINICAL CASES OF LIVESTOCK AND POULTRY IN COASTAL ANDHRA PRADESH

G. MOHANA SHEELA¹*, S. KRUPANIDHI^2
1Department of Biotechnology, Vignan University, Vadlamudi, Guntur, Andhra Pradesh-522213, India
²Department of Biotechnology, Vignan University, Vadlamudi, Guntur, Andhra Pradesh-522213, India
* Corresponding Author : mohana.sheelag@gmail.com

Received : 27-08-2015     Accepted : 21-09-2015     Published : 15-11-2015
Volume : 7     Issue : 5       Pages : 698 - 702
Int J Microbiol Res 7.5 (2015):698-702

Keywords : Staphylococcus aureus, Livestock, Poultry, Diseases, Biochemical tests, PCR
Academic Editor : Das Hemen, Lily Arsanti Lestari, Bhupesh P. Kamdi, Abdalnabi J. Abid, Sheetal S. K., Sarkar Pradip, S. S. Pathak, Azza S. Zakaria, Zaklina Cekovska, Dr Subhashree Sarangi, V. C. Ingle, Pazhanivel N., D. J. Talukdar , Gowda C. T. Chandre, Mayura R. Patil
Conflict of Interest : None declared
Acknowledgements/Funding : We thank the Associate Dean andthe Dept of Microbiology, NTR College of Veterinary Sciences, Gannavaram and Dr. P. Anand Kumar, Professor & Head, Department of Veterinary Microbiology, College of Veterinary Science, Sri Venkateswara Veterinary University,

Introduction: - Staphylococcus aureus is a panzootic gram positive pathogenic bacterium of mammalian species which produces a host of diseases in humans and animals. Since the organisms are transmissible from animals to human beings, its occurrence in animals possess a potential threat to humans. Aims and objectives: - A study was conducted in four coastal districts of Andhra Pradesh state Materials and Methods:- In the present study, 60 clinical samples of various animal diseases were collected from four coastal districts of Andhra Pradesh and were analysed for the presence of Staphylococcus aureus using biochemical tests like catalase, oxidase, Voges – Proskauer, and coagulase test and cultural characteristics like mannitol fermentation and haemolysis. Their presences were confirmed by PCR using species-specific primers for S. aureus. From the results, species wise, district wise and disease wise incidence of S. aureus is calculated Results: -The results indicate that 68% of bovine and 82% of poultry cases were positive for S. aureus. The district wise distribution varied from 56.53% to 100%. In disease wise, all the cases of bumble foot in poultry, 85.65% of gangrenous dermatitis in poultry and 68% of bovine mastitis cases were positive for S. aureus. Conclusion: - S. aureus is a potential threat due to its presence among animal diseases, which may be of zoonotic importance to in contact human beings. Further studies are required for the identification of antibiotic resistance pattern and mechanisms of antibiotic resistance of the organism.