CHARACTERIZATION OF INDIGENOUS BACILLUS COAGULANS ISOLATED FROM CATTLE AND BUFFALO MILK

LOPAMUDRA HALDAR¹*, D.N. GANDHI², DEBASIS MAJUMDAR³, SACHINANDAN DE^4
1Faculty of Science & Technology, ICFAI University, Agartala, Tripura- 799210, India
²Dairy Microbiology Division, National Dairy Research Institute, Karnal- 132001, Haryana, India
³Departments of Agricultural Statistics, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, Nadia- 741252, West Bengal, India
⁴Animal Biotechnology Centre, National Dairy Research Institute, Karnal- 132001, Haryana, India
* Corresponding Author : mohor7@gmail.com

Received : 26-09-2015     Accepted : 05-10-2015     Published : 15-11-2015
Volume : 7     Issue : 5       Pages : 686 - 691
Int J Microbiol Res 7.5 (2015):686-691

Keywords : Single strand conformational polymorphism, Bacillus coagulans, Bacillus pumilus, Bacillus subtilis.
Academic Editor : Tarek Mohamed Abdel Ghany, Kedar Nath
Conflict of Interest : None declared
Acknowledgements/Funding : The authors acknowledge the financial assistance and necessary supports provided by the Director of National Dairy Research Institute, Karnal, Haryana, India to carry out the study. The first author wishes to express her sincere thanks to Dr. Rameshwar Si

Spore forming and lactic acid producing Bacillus species, because of their heat stability and ability to survive the gastric barrier, are being attractive and gaining recognition for use as probiotics in human diets and animal feeds as well as in registered medicines. In the present study, we attempted to isolate Bacillus strains with special reference to Bacillus coagulans from milk, soil and tomato sources. Thirty-six representative isolates were characterized according to their morphological, biochemical properties, single strand conformational polymorphism (SSCP) banding patterns and partial 16S rRNA gene sequences. A wide range of polymorphism or heterogeneity within the species was recorded. Thirty-two isolates were identified as Bacillus coagulans (n= 32), while each two isolates were identified as Bacillus pumilus (n= 2) and Bacillus subtilis (n= 2) of which mostly were isolated from cattle and buffalo milk.