ISSN : 0975-5276
EISSN : 0975-9174
MANJEET KAUR1*, RAJENDRA SINGH2, MANDHAN RISHI PAL3, INDU SHARMA4
1ICAR-Indian Institute of Wheat and Barley Research, Karnal, Haryana, India,
2ICAR-Indian Institute of Wheat and Barley Research, Karnal, Haryana, India
3Department of Biotechnology, Kurukshetra University, Kurukshetra, India
4ICAR-Indian Institute of Wheat and Barley Research, Karnal, Haryana, India
* Corresponding Author : rajenderkhokhar@yahoo.com
Received : 15-09-2015 Accepted : 06-10-2015 Published : 15-11-2015
Volume : 7 Issue : 5 Pages : 679 - 682
Int J Microbiol Res 7.5 (2015):679-682
Keywords : Ustilago maydis, Tilletia indica, Simple sequence repeats, Cross transferability, Polymorphism
Academic Editor : Madhuri S. Bhandwalkar, Dr. Neeraj Dilbaghi
Conflict of Interest : None declared
Acknowledgements/Funding : The authors are grateful to MS Saharan, ICAR-Indian Institute of Wheat and Barley Research, Karnal and Ritu Bala, Punjab Agricultural University, Ludhiana for providing the inoculum to carry out the present study.
Simple sequence repeats (SSRs) or microsatellites are one of the valuable sources for genetic markers because of their abundance and inherent potential for determining extensive allelic variation in the genomes. A total of 1038 SSRs were detected in 12.4 Mb gene sequence data of Ustilago maydis in the present survey. The distribution pattern of different SSR motifs provides the evidence of greater accumulation of trinucleotide (63.1%) followed by dinucleotide (20%) and hexanucleotide (10.5%) in U. maydis. Among trinucleotide repeats, CAG repeats were more frequent while TC dinucleotide repeats were found to be predominant in U. maydis. Further, 14 simple sequence repeats (SSR) primers were designed based on genes responsible for pathogenicity in U. maydis. In terms of cross species transferability, a low level of transferability of SSRs was detected, and only 4 potential markers that can be used to fingerprint 18 isolates of Tilletia indica were identified