MOLECULAR DETECTION AND CAPSULAR TYPING OF Pasteurella multocida ISOLATED FROM CATTLE AND BUFFALO BY MULTIPLEX PCR ADJOINING AREA OF MATHURA, UP, INDIA

CHOWDHURY B.¹, QURESHI S.D.², VARSHNEY P.³, KHAN S.⁴, BHATIA A.K.^5
1Department of Microbiology & Immunology, College of Veterinary Sciences and Animal Husbandry, DUVASU, Mathura- 281 001, UP, India.
²Epidemiolgy Section, Indian Veterinary Research Institute, Izatnagar- 243 122, UP, India.
³Department of Microbiology & Immunology, College of Veterinary Sciences and Animal Husbandry, DUVASU, Mathura- 281 001, UP, India.
⁴Division of Animal Genetics, Indian Veterinary Research Institute, Izatnagar- 243 122, UP, India.
⁵Department of Microbiology & Immunology, College of Veterinary Sciences and Animal Husbandry, DUVASU, Mathura- 281 001, UP, India.

Received : 05-11-2013     Accepted : 18-12-2013     Published : 21-12-2013
Volume : 1     Issue : 2       Pages : 41 - 43
World Res J Biotechnol 1.2 (2013):41-43

The study was focused on to assess the rapid detection and typing of P. multocida isolated from HS affected cattle and buffaloes in and around Mathura, Uttar Pradesh (India). Ten P. multocida were recovered from the HS cases and were confirmed as P. multocida by species specific PM-PCR, further subjected to phenotypic and genotypic characterization. The multiplex capsular PCR has typed all of them into serogroup B. These PCR based techniques were specific and sensitive for rapid detection and typing of P. multocida isolates. The results indicated that PM-PCR could be used for rapid detection and epidemiological investigations of HS. The multiplex capsular PCR can be useful for serogrouping of P. multocida isolates.