ISSN : 0975-3079
EISSN : 0975-9190
Sheikh I.A.1, Charde M.S.2, Kasture A.V.3
1Department of Pharmaceutical Chemistry, J. L.Chaturvedi College of Pharmacy, Hingna MIDC, Nagpur-16, India
2Department of Pharmaceutical Chemistry, J. L.Chaturvedi College of Pharmacy, Hingna MIDC, Nagpur-16, India
3Department of Pharmaceutical Chemistry, J. L.Chaturvedi College of Pharmacy, Hingna MIDC, Nagpur-16, India
Received : - Accepted : - Published : 21-12-2009
Volume : 1 Issue : 2 Pages : 11 - 19
Int J Pharmaceut Anal 1.2 (2009):11-19
DOI : http://dx.doi.org/10.9735/0975-3079.1.2.11-19
Keywords : Flupenthixol Hydrochloride, Acetonitrile, pH, Eurospher, intersil, recovery, validation
Conflict of Interest : None declared
Acknowledgements/Funding : The authors thank Biocon Laborateries
Bangalore for the sample of Flupenthixol
Hydrochloride.The author also pays his
indebtness for Principal J L Chaturvedi
College of Pharmacy Nagpur for providing
Laboratory Facilities
A simple, selective, rapid, precise and economical reverse phase HPLC method has been developed for estimation of Flupenthixol Hydrochloride in pharmaceutical dosage forms. The method was carried out on a Eurospher C18 (250 cm×4 mm) column with precolumn and a mobile phase consisting of Acetonitrile:Water (pH 3.0) (50:50 v/v) at a flow rate of 1 ml/min. Detection was carried out at 229nm. Acetonitrile was used as an internal standard. The retention time of Flupenthixol HCl was found out to be 3.98. The developed method was validated in terms of accuracy, precision, linearity, limit of detection, limit of quantization and solution stability. The proposed method can be used for the estimation of these drugs in combined dosage forms.
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