ISOLATION OF 1, 2 DI-SUBSTITUTED IDOPYRANOSE FROM VITEX NEGUNDO AND ITS EFFECTS ON DIABETIC RATS

Manikandan R.¹*, Sundaram R.², Srinivasan P.³*, Beulaja S.⁴, Arulvasu C.⁵*
¹Department of Animal Health and Management, Alagappa University, Alagappapuram, Karaikudi, Tamilnadu, India
²Department of Zoology, University of Madras, Guindy Campus, Chennai 600 025, Tamil Nadu, India
³Department of Bioinformatics, Alagappa University, Alagappapuram, Karaikudi, Tamilnadu, India
⁴Department of Zoology, University of Madras, Guindy Campus, Chennai 600 025, Tamil Nadu, India
⁵Department of Zoology, University of Madras, Guindy Campus, Chennai 600 025, Tamil Nadu, India
* Corresponding Author : carulvasu@gmail.com

Received : -     Accepted : -     Published : 21-12-2009
Volume : 1     Issue : 2       Pages : 4 - 10
Int J Pharmaceut Anal 1.2 (2009):4-10
DOI : http://dx.doi.org/10.9735/0975-3079.1.2.4-10

Keywords : Vitex negundo, Verbanaceae, anti-diabetic compound, idopyranose, diabetes, Wistar albino rats
Conflict of Interest : None declared

The aim of this study was to investigate the protective effect of plant compound idopyranose is evaluated against streptozotocin-induced diabetics Wistar rats. The animals were divided into four groups consisting of 6 rats each. Group I contained the normal control were treated with olive oil, group II was served as a negative control (diabetic control), group III comprising normal rats were administered with idopyranose alone for 21 days and group IV diabetic rats were administered with idopyranose (50 mg /kg body wt/ day orally) respectively. Diabetic rats were given idopyranose isolated from the plant, Vitex negundo showed significant reduction in blood glucose, alanine aminotransaminase and aspirate aminotransaminase activities. Serum urea, creatinine and cholesterol were also significantly reduced when compared to diabetic control. Apart from these parameters, idopyranose showed improvement in parameters like body weight, lipid profile such as triglyceride, high density lipoprotein (HDL) and low density lipoprotein (LDL). In diabetic rats changes like coarsening of acinar cells of endoplasmic reticulation, destruction of beta cells and alternation in their secretary function were observed in the histomorphology of the pancreas. The changes like dilation of vein, loss of unusual concentric arrangement of hepatocytes and liver fibrosis were observed in the liver. Thickening of tubules and expansion of glomerulus were observed in kidneys. All these altered parameters were reversed close to normal condition by the treatment of idopyranose. According to biochemical and histological results obtained, it can be concluded that the idopyranose helps in regeneration of damaged pancreas and protects pancreatic ß cells and hyperglycemic nature against streptozotocin-induced diabetes.

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