MALDI-TOF based Metabolomic approach

Kapoor R.A.¹, Ladak S.S.², Gomase V.S.^3
1Padmashree Dr. D.Y. Patil University, Navi Mumbai, 400614, India
²Padmashree Dr. D.Y. Patil University, Navi Mumbai, 400614, India
³School of Technology, S.R.T.M. University, Sub-Centre, Latur, 413512, India

Received : -     Accepted : -     Published : 21-12-2009
Volume : 1     Issue : 2       Pages : 44 - 46
Genetics 1.2 (2009):44-46
DOI : http://dx.doi.org/10.9735/0975-2862.1.2.44-46

Keywords : Metabolomics, MALDI-TOF, Mass Spectrometry
Conflict of Interest : None declared

Metabolomics is the study of metabolite profiles in biological and pathological samples (urine, plasma, saliva, blood fat biopsies, plant cells, bacteria viruses and fungi. The metabolome is the most predictive phenotype: consequently, the comprehensive and quantitative research study of metabolites is a desirable tool for diagnosing disease, Identifying new therapeutic targets and enabling appropriate treatments. In addition to other Mass Spectrometry (MS) methodologies used for determining the localization of small molecules in tissue, MALDI-TOF/TOF can also be used to detect drugs and their metabolites in tissue without the burden of matrix interference.

[1] Rohit Shroffa, Lubomír Rulíšekb, Jan Doubskýa and Aleš Svatoša. (2009) PNAS, 25:10092-10096  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[2] Roessner U. et al. (2001) Plant Cell 13, 11– 29  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[3] Nicholson J.K. et al. (1999) Xenobiotica 29, 1181–1189  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[4] Robert Hall, Mike Beale, Oliver Fiehn, Nigel Hardy, Lloyd Sumner and Raoul Bino (2002) Plant Cell; 14(7): 1437–1440  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[5] Ruelle V., Moualij B. E., Zorzi W., Ledent P., Pauw E. D. (2004) Rapid Commun. Mass Spectrom. 18, 2013–2019  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[6] Singh O.V. (2006) Proteomics 6, 5481–5492  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[7] Wikoff W.R., Anfora A.T., Liu J., Schultz P.G., Lesley S.A., Peters E.C., Siuzdak G. (2009) Proc Natl Acad Sci U S A, 106(10):3698-703  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[8] Wikoff W.R., Pendyala G., Siuzdak G., Fox H.S. (2008) J Clin Invest. 118(7):2661-9  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[9] Monti J., Fischer J., Paskas S., Heinig M., Schulz H., Gösele C., Heuser A., Fischer R., Schmidt C., Schirdewan A., Gross V., Hummel O., Maatz H., Patone G., Saar K., Vingron M., Weldon S.M., Lindpaintner K., Hammock B.D., Rohde K., Dietz R., Cook S.A., Schunck W.H., Luft F.C., Hubner N. (2008) Nat Genet. 40(5):529-37  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[10] Wang Y., Tao Y., Lin Y., Liang L., Wu Y., Qu H., Liang T., Cheng Y. (2009) Anal Chim Acta. 633(1):65-70  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[11] De Luca V. and St. Pierre B. (2000) Trends Plant Sci. 5, 168–173  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[12] Ji-Na Wang, Ying Zhou, Tong-Yu Zhu, Xiangdong Wang and Yin-Long Guo(2008) J. Proteome Res.7 (8), pp 3597– 3601  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[13] Li Y., Shrestha B., Vertes A. (2008) Anal Chem 80:407–420.  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[14] Fiehn O. (2001) Comp. Funct. Genomics 2, 155–168 Kapoor RA, Ladak SS, Gomase VS  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus