Proteomics: Emerging analytical techniques

Gomase V.S.¹, Maurya Bharati D.², Pawar Shweta V.³, Chate Priya B.⁴, Kayarkar Namita A.⁵, Durgude Suvarna G.⁶, Boraste Amol⁷, Kadam Pallavi^8
1School of Technology, S.R.T.M. University, Sub-Centre, Latur, 413512, India
²V.E.S. College of Arts, Science and Commerce, Chembur, Mumbai-400 071, India
³V.E.S. College of Arts, Science and Commerce, Chembur, Mumbai-400 071, India
⁴Bhavan's College, Andheri (west), Mumbai-400 058, India
⁵V.E.S. College of Arts, Science and Commerce, Chembur, Mumbai-400 071, India
⁶V.E.S. College of Arts, Science and Commerce, Chembur, Mumbai-400 071, India
⁷Padmashree Dr. D.Y. Patil University, Navi Mumbai, 400614, India
⁸Padmashree Dr. D.Y. Patil University, Navi Mumbai, 400614, India

Received : -     Accepted : -     Published : 21-12-2009
Volume : 1     Issue : 2       Pages : 17 - 24
Genetics 1.2 (2009):17-24
DOI : http://dx.doi.org/10.9735/0975-2862.1.2.17-24

Keywords : Proteomics, imaging MS, 2D-DIGE, MudPIT, SELDI, MALDI TOF, Capillary electrophoresis
Conflict of Interest : None declared

Genomic and proteomic approaches together promise to reveal a multidimensional view of a biological system. As compared to Genomic approaches, Proteomic studies have their fair amount of limitations. The technical issues troubling proteomic approaches include gel-to-gel reproducibility, biases toward identifying similar proteins in unrelated proteomic studies, and reliability of protein extraction methods. Many types of information cannot be obtained from the study of genes alone because proteins not genes are responsible for the phenotypes of cells. Mechanisms of disease, aging and the effects of the environment cannot be explained solely by the genome because through the study of proteins can protein modifications be analyzed and the targets of drugs of identified.

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