Title |
WHITEFLY TRANSMITTED YELLOW MOSAIC DISEASE, SEVERE THREAT TO COWPEA PRODUCTION IN ASSAM, INDIA |
| World Res J Biotechnol Vol:3 Iss:1 (2016-08-28) : 53-56 |
Authors |
SANJEEV KUMAR, LINGARAJ SAHOO, BHABEN TANTI |
Published on |
28 Aug 2016 Pages : 53-56 Article Id : BIA0003077 Views : 1018 Downloads : 434 |
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Abstract |
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Open Access | Research Article
Yellow mosaic diseases (YMD) in economically important legumes across Southern Asia are majorly caused by four species of geminiviruses (genus Begomovirus, family Geminiviridae). YMD is transmitted by whitefly and causes severe loss to a number of important grain legumes, a rich source of dietary protein. The viruses have limited host range to plants of the family Fabaceae. The efforts to reduce the losses are hampered by limited availability of natural resistance sources and the lack of durable resistance. There exists active genetic interaction between these begomoviruses of the legumes, in the form of both component exchange and classical recombination, but very few studies are there on interaction with viruses that infect other plants. Genetic isolation indicates viruses infecting legumes evolve independently of the begomoviruses of other plant families. This has clear implications for the requirement to develop resistance in legumes, which holds the promise of durability. In our study, we surveyed in 4 districts of Assam, India, at various locations, for severity of viral diseases in cowpea. The morphometric appearances of viral disease in cowpea leaf samples in the field were collected. The genomic DNA were extracted and PCR performed for the detection of viral DNA. The PCR products were cloned and sequenced. The full length obtained sequences were analyzed using NCBI-BLAST and ClUSTL-W program for identification of virus.
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