This study was conducted for the first time in AL-Najaf / Iraq, and the study included the preparation of a local inactivated vaccine (whole and subunit). The number of cases infected with seasonal influenza virus was 647 case out of one thousand suspected case. How were distributed into 11 groups. Seasonal influenza virus was detected by three diagnostic methods (plasma pH, rapid test device and real time PCR). The present study reflected that the most diagnosed cases infected with seasonal influenza virus during the period extended from 03/26/2012 up to 04/30/2013 represented by were type A (H3N2) of (283). Whereas H1N1 (148) case, H3N2+H1N1, (56) case, H1N1 + H3N2 + B were (30) case. While only 130 case infected with type B.

This research aimed to investigate the serological relationship between two Egyptian cyanophage isolates (virulent and temperate) infecting Anabaena sp. A simple purification procedure, based on ultracentrifugation of the clarified lysates was applied, and diagnostic antisera were prepared by immunizing New-Zealand (NZ) rabbits using the purified preparations. Ouchterlony and micropreciptin tests were performed to check the serological relationship as well as discontinuous non-denaturing gel analysis. Molecular analysis of the amino acid sequences of coat protein genes was applied using DNA star lasergene 10 suite. The purified preparations revealed A260/A280 ratios of 1.4 and 1.1, concentration was 1.005 and 0.58 mg/ml, and precipitation dilution end point of 1/32 and 1/16 for virulent and temperate isolate respectively. The purified suspensions (7x 10⁴ and 5x 10⁴ pfu/ml, estimated by plaque assay, for virulent and temperate isolate respectively) were used to obtain the specific antisera. The purified preparations are found immunogenic, producing specific polyclonal antibodies. Data indicated a serological relationship between the two isolates which was discussed on the basis of discontinuous non-denaturing gel electrophoresis analysis and bioinformatic analysis of coat protein genes i.e antigenic indexes, secondary,3D and space fill structures.