The present study was undertaken to evaluate the protective effect of ionized selenium (ISe) and selenium Nano-particles (SeNps) against bisphenol A (BPA) induced hepatotoxicity and nephrotoxicity in experimental rats. The selected way of application for the study was oral gavages for 8 consecutive weeks. Adult male albino rats were randomly divided into six equal groups. The first one was served as a control and administrated the corresponding dose of corn oil, whereas the remaining groups were respectively treated with bisphenol A (150mg/Kg. b.wt), bisphenol A plus ISe (3mg/kg. b.wt), bisphenol A plus SeNps (2mg/Kg. b.wt), ISe and SeNps. The exposure of rats to BPA induced hepatotoxicity and nephrotoxicity resulted in increased transaminases (AST, ALT) and alkaline phosphatase (ALP)activities. The concentrations of total cholesterol, blood urea nitrogen and creatinine were also elevated. BPA induced significant decrease in the GSH level and catalase activity while lipid peroxidation (LPO) was significantly increased in both liver and kidney homogenates. In the same time, BPA caused significant increase in DNA fragmentation in liver and kidney homogenates of exposed rats. Marked histopathological alterations were revealed in both liver and kidney tissues. The co-administration of ISe and SeNps attenuated the biochemical parameters cited above as well as the changes in DNA fragmentation. Liver and kidney histopathological studies have confirmed the changes observed in biochemical parameters and proved the beneficial role of selenium preparations. In conclusion, the use of SeNps appeared to be more significant than ISe in attenuating the undesirable effects of BPA.