Aim: Chronic suppurative otitis media is a significant health problem especially in underdeveloped countries. Pseudomonas aeruginosa is very common causes of inflammation with distinct tendency for multi-resistance development, which can cause significant and even life-threatening complications. This study aims to determine the microbial profile and sensitivity of Pseudomonas aeruginosa isolates to antibiotics, which would greatly help in the treatment of disease, preventing the development of complications and the development of pathogens resistant to certain antibiotics. Methodology: The standard microbiological procedures were followed for isolation, culture and antimicrobial assay. Results: Pseudomonas aeruginosa was present in 27.18% and isolates showed full susceptibility to Imipenem and Ceftizidim (100%), meaningly susceptibility to Ciprofloxacillin (92.68%), and slightly less to Cefoperazone (78.95%) and Piperacillin (76.92%). There was also relatively considerable resistance to aminoglycosides Gentamycin (50%) and Amikacin (53.85%), and complete resistance to Ampicillin, Amoxicillin-clavulanate, Cephalexin and Cefuroxime. Conclusion: Given the high affinity multi-resistance of Pseudomonas aeruginosa it is necessary to continuously and periodically evaluate the aetiological agents of chronic suppurative otitis media and their susceptibility to antibiotics.

Background: Vagina is a microbiological museum where most types of aerobic and anaerobic organisms are present as normal commensals but when there is imbalance in the flora, the individual experiences abnormal vaginal discharge. It’s one of worrisome problems that all females in reproductive age group come across at least once in their life. As it causes few morbid complications such as infertility, pelvic inflammatory disease, endometriosis, cuff cellulitis, urethral syndrome, pregnancy loss, preterm labour; increased risk of other STDs, it’s important to identify specific etiological agents and their sensitivity for commonly used antibiotics. Methods: Two hundred vaginal swabs from clinically suspected vaginitis cases taken were used to prepare saline wet mount, pH estimation, Gram stain, Methyl-violet stain, KOH mount and whiff/amine test. The isolates were inoculated on Blood agar, MacConkey agar and Sabouraud dextrose agar and incubated aerobically at 370C for overnight. Organisms were then identified and antibiotic sensitivity test was performed. Results: Most common cause of the symptomatic vaginal discharge was Aerobic vaginitis accounting for about 32%. Commonest organisms were E. coli, P. aeruginosa and S. aureus. Most were sensitive to Amikacin except for P. aeruginosa, which was sensitive to imipenem and colistin. Vulvo vaginal-candidiasis, bacterial-vaginosis and trichomoniasis was seen in 14.5%, 14% and 6.5% of our cases respectively. Conclusion: Diagnosis cannot be only relied on clinical approach instead, Microbiological diagnosis is most important for specific treatment to avoid the complications of vaginitis & antibiotic resistance.